西安交通大学学报（医学版）2017，Vol.38Issue(5)：643-648,6.DOI:10.7652/jdyxb201705004
低温等离子体对小鼠皮肤伤口愈合的影响
Effect of low-temperature plasma on wound healing of mouse skin
摘要
Abstract
Objective To compare the different doses of low-temperature plasma (LTP) on wound healing in BALB/c mice so as to discuss the effects of the optimal dose of low-temperature plasma dealing with wound in mice and the acting mechanism of wound healing.Methods Adoptatmospheric pressure plasma jet discharged by the dielectric barrier was used to treat mouse skin wound.According to the processing time, the wounds were divided into 10s, 20s, 30s, 40s and 50s experimental groups, while naturally healing wounds served as negative controls and the wounds dealt with recombinant human epidermal growth factor served as positive controls.We recorded the wound size every day, observed the histopathological changes, the expression level of type Ⅰ collagen by immunofluorescence, and analyzed the composition of low-temperature plasma jet.Results The wounds with plasma treatment time of 10s, 20s, 30s, and 40s showed significant daily improvement and almost complete closure at days 12, 10, 7, 13, respectively.However, the wounds with plasma treatment time of 50s remained unhealed atday 14.The wounds in positive control group all healed, and the wound healing effect in positive control group could be achieved in 30s group.HE staining and immunofluorescence staining assays showed the optimal result of epidermal cell regeneration, granulation tissue hyperplasia, and collagen deposition in histological aspect at day 7 in 30 s group.The low-temperature plasma jet contained highly reactive free radicals of nitrogen and oxygen, which play an important role in wound healing process.Conclusion Appropriate doses of cold plasma can accelerate wound healing whereas over-doses of plasma can suppress wound healing.The process of wound healing may be related to reactive oxygen and nitrogen species in LTP.关键词
等离子体射流/BALB/c小鼠/创伤愈合Key words
plasma jet/BALB/c mouse/wound healing分类
医药卫生引用本文复制引用
蔡晶芬,刘进仁,任鸿斌,许桂敏,陈思乐,孙养信,石兴民,张冠军..低温等离子体对小鼠皮肤伤口愈合的影响[J].西安交通大学学报（医学版）,2017,38(5):643-648,6.基金项目
国家自然科学基金资助项目(No.81372076、51677146) （No.81372076、51677146）
陕西省科技计划项目(No.2010K16-04)Supported by the National Natural Science Foundation of China (No.81372076 and 51677146)and the Sci-Tech Project of Shaanxi Province(No.2010K16-04) （No.2010K16-04）
