西安交通大学学报（医学版）2017，Vol.38Issue(5)：649-654,6.DOI:10.7652/jdyxb201705005
MiR-26a/b在AngⅡ导致的高血压血管重塑中的作用
Role of miR-26a/b in AngⅡ-induced hypertensive vascular remodeling
摘要
Abstract
Objective To detect the expression of miR-26a/b in the aorta and serum of mice so as to explore the role of miR-26a/b in vascular remodeling of hypertension.Methods C576L/BJ male mice were randomly divided into AngⅡ group and control group.Mini-osmotic pump was implanted subcutaneously into the back of mice, and the model of blood vessel remodeling in mice was established by continuous infusion of AngⅡ(2.0mg/kg·d).The mice in control group were injected with saline.Blood pressure was taken before the intervention and at 3, 5, 7, 10 and 14 days after the intervention.After 2 weeks, the mice were killed, the serum and aorta tissues were collected, and the expression of miR-26a/b was determined by RT-PCR.HE staining, Masson staining and immunohistochemistry were performed to observe changes in vascular morphology, fibrosis and protein expression.Results After the intervention, systolic blood pressure and diastolic blood pressure were significantly higher in AngⅡ group than in control group (P<0.05).HE staining showed that the vessel wall of AngⅡ group was thicker than that of control group.Masson staining showed more blue collagen deposition in the middle of aorta in AngⅡ group but no obvious collagen deposition in control group.RT-PCR showed that the expression of miRNA-26a/b in the serum and aorta of AngⅡ group was significantly lower than in control group (P<0.05).Immunohistochemistry indicated that the expressions of CTGF, collagen Ⅰ and collagen Ⅲ all increased after AngⅡ infusion (P<0.05).Conclusion MiR-26a/b, CTGF, collagen Ⅰ and collagen Ⅲ may be involved in AngⅡ-induced vascular remodeling in hypertension.MiR-26a/b may be a new therapeutic target of vascular remodeling in hypertension.关键词
高血压/血管重塑/microRNA/miRNA-26a/b/细胞外基质Key words
hypertension/vascular remodeling/microRNA/miRNA-26a/b/extracellular matrix分类
医药卫生引用本文复制引用
张益珊,袁苗,黄园,王巧竹,张文倩,高登峰..MiR-26a/b在AngⅡ导致的高血压血管重塑中的作用[J].西安交通大学学报（医学版）,2017,38(5):649-654,6.基金项目
国家自然科学基金资助项目(No.81570382)Supported by the National Natural Science Foundation of China(No.81570382) （No.81570382）
