西安交通大学学报（医学版）2017，Vol.38Issue(5)：744-748,5.DOI:10.7652/jdyxb201705025
补骨脂素对乳腺癌干细胞拓扑异构酶Ⅱα表达的影响
Effect of psoralen on TopoIIα expression in breast cancer stem cells
摘要
Abstract
Objective To explore the effect of psoralen on topoisomerase IIα(TopoIIα) expression of breast cancer stem cells.Methods CD44+CD24-/low breast cancer stem cells were sorted from MCF-7/ADR by magnetic-activated cell sorting(MACS).We observed the growth characteristics of these stem cells through optical microscope and detected the growth-inhibitory effects of psoralen on breast cancer stem cells by CCK-8 assay and IC50 of adriamycin and adriamycin combined with psoralen to calculate the reversal index.The mRNA and protein expressions of Topo IIα were detected using RT-PCR and Western blot, respectively.Results Under the optical microscope, breast cancer stem cells presented spheres.IC10 and IC20 of psoralen on breast cancer stem cells were (6.77±0.23)μg/mL and (10.36±0.21)μg/mL.IC50 of adriamycin and adriamycin combined with psoralen on breast cancer stem cells was (90.03±3.56)μg/mL and (21.47±0.82)μg/mL, the reversal index was 4.19.Psoralen significantly raised the expressions of Topo Ⅱα at mRNA and protein levels.Conclusion Psoralen reversed the resistance of adriamycin by increasing the gene and protein expressions of breast cancer stem cells Topo Ⅱα and the drug targets.关键词
补骨脂素/拓扑异构酶Ⅱα/耐药/干细胞/乳腺癌Key words
psoralen/TopoⅡα/drug resistance/stem cell/breast cancer分类
数理科学引用本文复制引用
许乘凤,王晓红,花义同,程凯,邹伟伟,刘健,张英哲,杨振林..补骨脂素对乳腺癌干细胞拓扑异构酶Ⅱα表达的影响[J].西安交通大学学报（医学版）,2017,38(5):744-748,5.基金项目
国家自然科学基金资助项目(No.81173601、No.30972932) （No.81173601、No.30972932）
滨州市科技发展计划项目(No.2015ZC0301) （No.2015ZC0301）
滨州医学院科研计划与科研启动基金(No.BY2014KYQD36) （No.BY2014KYQD36）
山东省高等学校科技计划项目(No.J15LL51) （No.J15LL51）
山东省自然科学基金(No.ZR2014HQ020)Supported by the National Natural Science Foundation of China(No.81173601and30972932),Projects of Binzhou Technology Development Program (No.2015ZC0301),Scientific Research Staring Foundation of Binzhou Medical College (No.BY2014KYQD36),and the Scienceand Technology Program of Universities in Shandong Province(No.J15LL51),and Natural Science Foundation of Shandong Province(No.ZR2014HQ020) （No.ZR2014HQ020）
