现代妇产科进展2017，Vol.26Issue(9)：641-646,6.DOI:10.13283/j.cnki.xdfckjz.2017.09.030
TGF-β1调节miR-99家族对卵巢癌细胞恶性行为的影响
TGF-β1 affects malignant behaviors of ovarian cancer cell via regulating miR-99 family
摘要
Abstract
Objective:To investigate the effect of TGF-β1 on biological behaviors of malignance in ovarian cancer cell by regulating miR-99 family. Methods:Ovarian cancer cell line A2780 and SKOV3 were treated with TGF-β1 or transfected with miRNA mimic or inhibitor of miR-99 family. Then the expression level of miR-99 family was quantified by RT-PCR. The proliferation ability was detected by CCK-8 cell counting test. The migration capacity was exam-ined by wound healing assay. The invasion capacity was determined by transwell assays. Re-sults:Compared to the blank control group,there was significant upregulation of miR-99 family level after being treated with TGF-β1 (P<0. 01). Compared to the blank control group,the a-bility of proliferation,migration and invasion was also enhanced after being treated with TGF-β1 (P<0. 05);the ability of proliferation was weakened (P<0. 05),while the ability of migration and invasion was significantly strengthened ( P<0 . 05 ) after transfected with miR-99 family mimic;the ability of proliferation was enhanced ( P<0 . 05 ) while the ability of migration and invasion was significantly crippled (P<0. 05) after transfected with miR-99 family inhibitor. Conclusion:The proliferation,migration and invasion ability of ovarian cancer cell A2780 and SKOV3 were promoted by TGF-β1 via miR-99 family,which could be used in predicting the malignancy of ovarian cancer.关键词
TGF-β/MiR-99家族/卵巢癌/增殖/迁移/侵袭Key words
TGF-β1/miR-99 family/Ovarian cancer/Proliferation/Migration/Invasion分类
医药卫生引用本文复制引用
陈雨龙,宋克娟,曹佃霞,吕腾,于晓敏,姚勤..TGF-β1调节miR-99家族对卵巢癌细胞恶性行为的影响[J].现代妇产科进展,2017,26(9):641-646,6.基金项目
国家自然科学基金资助项目(No:81100697) （No:81100697）
山东省博士基金资助项目(No:BS2001SW003) （No:BS2001SW003）
