中国组织工程研究2017，Vol.21Issue(22)：3476-3482,7.DOI:10.3969/j.issn.2095-4344.2017.22.006
多孔纳米复合材料Se@SiO2治疗激素性股骨头坏死
Porous Se@SiO2 nanocomposites for treatment of steroid-induced osteonecrosis of the femoral head
摘要
Abstract
BACKGROUND:Steroid-induced osteonecrosis of the femoral head (SONFH) is a common bone disease characterized as high morbidity and poor prognosis,but the pathogenesis is unclear.Oxidative stress treatment is closely related to the occurrence and development of SONFH,and has tremendous potential in the treatment of SONFH,which can be realized by Nano-Se.OBJECTIVE:To observe the protective effect of porous Se@SiO2 nanocomposite on chondrocytes by antioxidant stress,and to further explore its mechanism of protection and treatment of SONFH.METHODS:(1) In vitro experiment:The rat chondrocytes were isolated,cultured and identified.Then,the chondrocytes were cultured with porous Se@SiO2 nanocomposite to suppress the production of reactive oxygen species (ROS).(2) In vivo experiment:A total of 36 rats were randomly divided into three groups.Steroid-induced group and experimental group were treated with intraperitoneal injection of lipopolysaccharide and intramuscular injection of methylprednisolone to induce SON FH models.Seven days after modeling,the experimental group was intraperitoneally injected with porous Se@SiO2 nanocomposite.No intervention was done in control group (blank control).At 8 weeks after modeling,rat bilateral femoral heads were taken for hematoxylin-eosin staining and Micro-CT scanning.RESULTS AND CONCLUSION:Results from the ROS detection and TUNEL apoptosis tests showed that the level of ROS in the chondrocytes was significantly reduced after intervention with Se@SiO2 (P ＜ 0.05).Micro-CT scanning findings showed that the bone mineral density,bone volume,bone area/bone volume,trabecular number,trabecular thickness,and trabecular separation in the steroid-induced and experimental groups were significantly different from those in the control group (P ＜ 0.05).Hematoxylin-eosin staining results showed smooth femoral head,normal bone cells,chondrocytes and trabecular bone,as well as few empty bone lacunae and fat cells in the control group,while in the steroid-induced group,there was bone trabecular fracture,fat cell hypertrophy fusion,a large number of empty bone lacunae and obvious osteonecrosis.These manifestations were significantly improved in the experimental group.To conclude,the porous Se@SiO2 nanocomposite has good antioxidative stress ability,suppresses the ROS production and exerts therapeutic effects on SONFH.关键词
生物材料/骨生物材料/激素性股骨头坏死/氧化应激/软骨细胞/纳米材料/纳米Se/活性氧/MicroCT/缓释/多孔/大鼠/国家自然科学基金分类
医药卫生引用本文复制引用
牛科润,杨孟恺,马春辉,俞银贤,滕松松,王谦,易诚青..多孔纳米复合材料Se@SiO2治疗激素性股骨头坏死[J].中国组织工程研究,2017,21(22):3476-3482,7.基金项目
国家自然科学基金面上项目(81371979) （81371979）
上海交通大学“医工交叉研究基金”项目(YG2015QN16)the National Natural Science Foundation of China,No.81371979 （YG2015QN16）
the Medical Cross Research Fund of Shanghai Jiao Tong University,No.YG2015QN16 （）
