中国组织工程研究2017，Vol.21Issue(22)：3488-3493,6.DOI:10.3969/j.issn.2095-4344.2017.22.008
制备缓释骨形态发生蛋白2的丝素蛋白/壳聚糖/纳米羟基磷灰石生物支架
Preparation of silk fibroin/chitosan/nano-hydroxyapatite scaffold for sustained release of bone morphogenetic protein-2
摘要
Abstract
BACKGROUND:Bone morphogenetic protein-2 (BMP-2) is a key to bone formation and repair.However,it has some disadvantages such as easy to lose and degrade and difficult to sustain continuous effect.OBJECTIVE:To study the preparation and properties of silk fibroin/chitosan/nano-hydroxyapatite (SF/CS/nHA) scaffold loading BMP-2.METHODS:After silk degumming,dissolution and purification,2％ SF solution was obtained.BMP-2 was dissolved in 2％ CS solution,and then fully mixed with equal volume of SF solution and proper amount of nHA.At last,the SF/CS/nHA scaffold loading BMP-2 was prepared using freeze-drying method as experimental group.The SF/CS/nHA scaffold was soaked in the BMP-2 solution as control group.The scaffold porosity was measured by Archimedes method,the surface morphology of the scaffold was observed by scanning electron microscope,the compressive strength was measured by universal testing machine.Scaffolds in the two groups were soaked in PBS,and the release of BMP-2 was measured by ELISA method at different time points.RESULTS AND CONCLUSION:(1) The scaffolds in the two groups had irregular porous structure,interconnected pores and uneven pore wall.There was no significant difference between the two groups in mean pore diameter,porosity and maximum compressive strength.(2) On the 1st day,the release rate of BMP-2 was 4.63％ in the experimental group,and the release curve increased slowly.After 28 days,the release curve of BMP-2 was transferred to the plateau stage.But in the control group,the release rate of BMP-2 on the 1stday was 58.84％,and it was a significant initial burst release.The release curve increased rapidly,and was transferred to the platform stage on the 10th day.The release rate of BMP-2 release was significantly different between the two groups at days 1,2,4,10 (P ＜ 0.05).These results show that the SF/CS/nHA scaffold loading BMP-2 could sustainably and slowly release BMP-2.关键词
生物材料/缓释材料/骨形态发生蛋白2/丝素蛋白/壳聚糖/纳米羟基磷灰石/药物缓释/生物支架/骨诱导/骨组织工程/国家自然科学基金分类
医药卫生引用本文复制引用
黄文良,叶鹏,莫刚,田仁元,马立坤,阮世强,徐林,邓江..制备缓释骨形态发生蛋白2的丝素蛋白/壳聚糖/纳米羟基磷灰石生物支架[J].中国组织工程研究,2017,21(22):3488-3493,6.基金项目
遵义市科技创新团队项目(遵市科合社字[2012]16号) （遵市科合社字[2012]16号）
贵州省科技攻关发展项目(黔科合SY字[2013]3017) （黔科合SY字[2013]3017）
贵州省自然科学技术重点基金(黔科合基础[2016]1420号) （黔科合基础[2016]1420号）
国家自然科学基金(81660367)the Scientific Innovation Team Project of Zunyi City,No.[2012]16 （81660367）
the Scientific and Technological Research and Development Project of Guizhou Province,No.[2013]3017 （）
the Natural Science Foundation of Guizhou Province,No.[2016]1420 （）
the National Natural Science Foundation of China,No.81660367 （）
