云南农业大学学报（自然科学）2017，Vol.32Issue(4)：571-576,6.DOI:10.16211/j.issn.1004-390X(n).2017.04.001
普通野生稻(Oryza rufupogon)糖基转移酶基因OrGT1(t)的克隆与表达分析
Cloning and Expression Analyses of Glycosyltransferases Gene OrGT1 (t) in Oryza rufupogon
摘要
Abstract
[Purpose] In this study,the preliminary study on the glycosyltransferase gene OrGT1 (t) in wild rice was carried out,which laid a solid foundation for the further exploration of the synthesis and function of xyloglucan in monocotyledonous plants.[Method] A glycosyltransferases gene OrGT1 (t) was cloned from Oryza rufupogon using homologous cloning methods.[Results] Sequence analysis revealed that the gene has a conservative Glyco-transf-34 domain,belonging to the Glyco-transf-34 gene falimy.Phylogenetic analysis showed that OrGT1 may be a xyloglucan xylosetransferase geneinvolve in xyloglucan synthesis.RT-qPCR analysis indicated that the OrGT1 expressed in all checked organs,including the root,stem,leaf and spike in O.rufupogon.But the transcriptional quantity was different among the organs,and there were the highest expression quantity in the stem and the lowest in the root.[Conclusion] The OrGT1 gene is a high degree of functional conservation in the evolutionary process,suggesting that the OrGT1 (t) gene may be a xyloglucanase gene involved in xyloglucan synthesis,the results lay a good foundation for further exploring the function of the OrGT1 gene.关键词
普通野生稻/糖基转移酶基因/基因克隆/实时荧光定量PCRKey words
Oryza rufupogon/glycosyltransferase gene/gene clone/real-time PCR分类
生物科学引用本文复制引用
罗碧,冯洁深,白和灵,刘超,赵锦龙,谭亚玲,徐津..普通野生稻(Oryza rufupogon)糖基转移酶基因OrGT1(t)的克隆与表达分析[J].云南农业大学学报（自然科学）,2017,32(4):571-576,6.基金项目
国家自然科学基金项目(31560300) （31560300）
云南省重点科技计划项目(2014BB016). （2014BB016）
