云南农业大学学报(自然科学)2017,Vol.32Issue(4):577-581,5.DOI:10.16211/j.issn.1004-390X(n).2017.04.002
氮素和Wx基因缺失对小麦淀粉生物合成的影响
Effects of Nitrogen and Wx Gene Deletion on Starch Biosynthesis in Wheat
摘要
Abstract
[Purpose] The effects of nitrogen fertilizer level and grain filling stage on the grain starch content of near isogenic Wx wheat lines were determined by different nitrogen application levels.[Method] Eight Wx near-isogenic wheat lines in Ningmai 14 were researched at no basal fertilizer,and 5 nitrogen levels including 0,150,300,450 and 600 kg/hm2 were fertilized at three-leaf period.The amylose,amylopectin and total starch contents of wheat grain were determined at 7,14,21,28 and 35 days after anthesis.[Results] The grain amylose,amylopectin and total starch contents were significantly affected by the Wx genotypes and grain filling period,while the nitrogen levels did not cause significant changes in content and composition of starch.Starch synthesis was speed up 10 d after anthesis till the end of filling period.[Conclusion] The amylose synthesis ability of three Wx genes was Wx-B1 > Wx-D1 > Wx-A1,and the comprehensive effect of Wx-A1 and Wx-D1 was the equivalent of Wx-B1.关键词
Wx近等基因系/氮素水平/淀粉/生物合成Key words
Wx near-isogenic wheat lines/nitrogen level/starch/biosynthesis分类
农业科技引用本文复制引用
李昆仑,孔治有,沙云,唐永生,程顺和,覃鹏..氮素和Wx基因缺失对小麦淀粉生物合成的影响[J].云南农业大学学报(自然科学),2017,32(4):577-581,5.基金项目
云南省科技计划项目(2015BB016) (2015BB016)
昆明市科技计划项目(2015-1-H-00308). (2015-1-H-00308)