云南农业大学学报（自然科学）2017，Vol.32Issue(4)：612-619,8.DOI:10.16211/j.issn.1004-390X(n).2017.04.008
槟榔江水牛Dmrt7基因克隆及分子特征、组织表达谱分析
Cloning, Molecular Characterization and Tissue Expression Profile of Binglangjiang Buffalo Gene Dmrt7
摘要
Abstract
[Purposes] As one of the members of Dmrt gene family,Dmrt7 gene affects the spermiogenesis of male animals.So far,few studies have been done related to the genetic basis of buffalo spermiogenesis.In this study,by employed Binglangjiang water buffalo as research object,the complete CDS sequence of water buffalo Dmrt7 gene was cloned,and its sequence characterization,tissue differential expression were assayed and analysed.[Methods] RT-PCR and functional bioinformatics methods were used.[Results] The results showed that the CDS of Binglangjiang buffalo Dmrt7 gene is 1 113 bp in length,which encodes a polypeptide composed of 370 amino acids.Buffalo Dmrt7 contains two conserved domains,viz.a DM and a DMRT-like domain.This protein was predicted a nuclear protein without signal peptide and transmembrane domain.Homology analysis showed that the amino acid sequence similarity of Dmrt7 between buffalo and other bovine species was more than 95％.Among 10 different tissues of adult buffalo,Dmrt7 gene was detected to only express in the testis of water buffalo,and its expression level was dominantly high.The polymorphism c.665A ＞ G was found in exon 6 of CDS of buffalo Dmrt7 gene which leads to a p.Q222R change.But this substitution was presumed to have no functional effect on buffalo Dmrt7 protein.[Conclusion] The results of this study will provide fundamental insights into the function of buffalo Dmrt7 gene.关键词
槟榔江水牛/Dmrt7基因/DM结构域/精子生成/遗传变异/生物信息学分析Key words
Binglangjiang buffalo/Dmrt7 gene/DM domain/spermatogenesis/expression profile/bioinformatics analysis分类
农业科技引用本文复制引用
伍姗,吉丽,张永云,杜平,康定富,哈福,苗永旺..槟榔江水牛Dmrt7基因克隆及分子特征、组织表达谱分析[J].云南农业大学学报（自然科学）,2017,32(4):612-619,8.基金项目
国家自然科学基金项目(31460582,30660024) （31460582,30660024）
云南省应用基础研究重点项目(2014FA032,2007C0003Z) （2014FA032,2007C0003Z）
云南省应用基础研究计划面上项目(2006C0034M,2010ZC243). （2006C0034M,2010ZC243）
