云南农业大学学报（自然科学）2017，Vol.32Issue(4)：678-684,7.DOI:10.16211/j.issn.1004-390X(n).2017.04.016
野生紫斑百合多倍体诱导研究
Studies on Polyploidy Induction of Wild Lilium nepalense D.Don
摘要
Abstract
[Purpose] To induce the polyploid and it was expected to obtain new polyploidy germplasm of wild Lilium nepalense.[Methods] The adventitious buds of wild L.nepalense were respectively treated with colchicines by soaking and mixing culture method,and the inducing effects of colchicine at different treatments,mass concentrations,and duration time were investigated.In addition,the variant plants were identified by morphological,stomatal and cytological observations.[Results] The soaking method was applied for polyploid induction,the optimum concentration of colchicine was 0.3 ％,and the optimum time duration was 12 h,with the mutagenic rate as 10.0 ％.When the mixing culture method was applied for chromosome doubling,the optimum concentration of colchicine was 0.7 ％,and the mutagenic rate was 46.67 ％.Through the identification,the tetraploid plants of wild L.nepalense were screened out.[Conclusion] Both of soaking and mixing culture method with colchicine could be used for polyploidy induction of wild L.nepalense,but the effect of mixing culture method with colchicine was better.关键词
紫斑百合/组织培养/秋水仙素/多倍体诱导Key words
Lilium nepalense D.Don/tissue culture/colchicines/polyploidy induction分类
农业科技引用本文复制引用
李旦,罗一然,韩国伟,张雪,吴英英,何承忠..野生紫斑百合多倍体诱导研究[J].云南农业大学学报（自然科学）,2017,32(4):678-684,7.基金项目
云南省环保专项资金项目(2014BI0011) （2014BI0011）
云南省教育厅重大专项项目(ZD2013014):云南省林学一流学科建设项目(51600625) （ZD2013014）
西南林业大学大学生创新基金项目(C16013,C15066). （C16013,C15066）
