中风与神经疾病杂志2017，Vol.34Issue(8)：718-720,3.
脑源性神经生长因子对mir124表达及兴奋性电流的影响
The effect of BDNF on levels of miRNA-124 and sEPSCs
摘要
Abstract
Objective To study the effect of brain derived neurotrophic factor (BDNF) in the pathogenesis from patients with MTLE.Methods The study was performed between April 2008 and October 2010.Surgically removed specimens were collected from the patients with MTLE.All patients gave written informed consent for research use of the biopsy materials.Surgically resected hippocampal were collected and immediately immersed in oxygenated ice-cold SACSF.The expression of Mir-124 was detected by RT-PCR and sEPSCs by patch-clamp.Results Expression of mir-124 was significantly decreased in Dentate Gyrus of MTLE compared with that of controls (P<0.01).BDNF increases frequency and amplitude of sEPSC (P<0.01).Conclusions The results indicate that BDNF significantly decreased the expression of mir-124 as in Dentate Gyrus from patients with MTLE.Our electrophysiological findings indicate that BDNF increase excitability on dentate granule cells.In conclusion,our work supports a role of BDNF in pathophisyology of MTLE and raises the possibility that interference with BDNF action may be a therapeutic method for patients of MTLE.关键词
脑源性神经生长因子/颞叶内侧癫痫/miRNA-124/自发性兴奋性突触后电流Key words
BDNF/mir-124/MTLE/sEPSCs分类
医药卫生引用本文复制引用
王凤军,王洪朋,单晶丽,邵正凯,姜杨薇,陈元元,宋婷婷,邱丽婷..脑源性神经生长因子对mir124表达及兴奋性电流的影响[J].中风与神经疾病杂志,2017,34(8):718-720,3.基金项目
黑龙江省教育厅面上项目(No.12541509) （No.12541509）
