中国防痨杂志2017，Vol.39Issue(8)：809-814,6.DOI:10.3969/j.issn.1000-6621.2017.08.005
84株广泛耐药结核分枝杆菌对新型氟喹诺酮类药物的耐药情况分析
Resistance analysis of 84 extensively drug-resistant Mycobacterium tuberculosis isolates against three new generation fluoroquinolones
宗兆婧 1荆玮 1霍凤敏 1董玲玲 1马异峰 1逄宇 1黄海荣1
作者信息
- 1. 101149 首都医科大学附属北京胸科医院 北京市结核病胸部肿瘤研究所 国家结核病临床实验室
- 折叠
摘要
Abstract
Objective To investigate the resistant status of extensively drug-resistant Mycobacterium tuberculosis isolates against new genratation fluroquinolones (FQs).Methods Extensively drug-resistant Mycobacterium tuberculosis (XDR-MTB) clinical isolates were collected from Beijing Chest Hospital between 2012-2014.Absolute concentration method was used for ofloxacin (Ofx) and levofloxacin (Lfx) susceptibility testing, while broth dilution method was used to detect the minimal inhibitory concentration (MIC) of the isolates against three FQs,including Lfx,moxifloxacin (Mfx) and gatifloxacin (Gfx).The quinolone resistance determining regions (QRDR) of gyrA and gyrB were sequenced for all the enrolled isolates.Results A total of 84 XDR-MTB isolates were enrolled.Among them,74 (74/84, 88.1%), 37 (37/84,44.0%) and 52 (52/84, 61.9%) isolates were resistant to Lfx, Mfx and Gfx according the cut-off criteria recommended by WHO, respectively.Mutations at 88, 90, 91 and 94 locus of gyrA were detected among 89.3% (75/84)of the enrolled isolates, and Asp94Gly accounting for high-level FQs resistance.All the strains had wild type gyrB.Conclusion Our findings demonstrated that new generation FQs resistance were very frequently observed among XDR-MTB isolates, and mutation within QRDR of gyrA was the main mechanism of FQs resistance.关键词
泛耐药结核病/分枝杆菌,结核/喹诺酮类/微生物敏感性试验/多药耐药相关蛋白质类/点突变Key words
Extensively drug-resistant tuberculosis/Mycobacterium tuberculosis/Quinolones/Microbial sensitivity tests/Multidrug resistance-associated proteins/Point Mutation引用本文复制引用
宗兆婧,荆玮,霍凤敏,董玲玲,马异峰,逄宇,黄海荣..84株广泛耐药结核分枝杆菌对新型氟喹诺酮类药物的耐药情况分析[J].中国防痨杂志,2017,39(8):809-814,6.
