中国感染控制杂志2017，Vol.16Issue(9)：798-803,6.DOI:10.3969/j.issn.1671-9638.2017.09.002
隐丹参酮对表皮葡萄球菌生物膜的抑制作用
Inhibitory effect of cryptotanshinone on biofilm of Staphylococcus epidermidis
摘要
Abstract
Objective To investigate the inhibitory effect of cryptotanshinone on different maturation stages of Staphylococcus epidermidis (S.epidermidis) biofilm.Methods The biofilm model of S.epidermidis was constructed in vitro, the timing of adhesion, accumulating, and maturation was determined;matrix quantity, bacterial metabolism, microstructure of biofilm were detected with semi-quantitative adhesion test, XTT assay, and scanning electron microscope(SEM) respectively.Results The timing of adhesion, accumulating, and maturation of S.epidermidis biofilm were 6h, 24h,and 48h respectively;in adhesion period, cryptotanshinone at the concentration of 128μg/mL and 32μg/mL could both obviously reduce the matrix and kill bacteria inside biofilm, difference was statistically significant(P<0.05),inhibitory effect of 128μg/mL cryptotanshinone was better than 32μg/mL (P<0.05), the microstructure was destroyed by both concentrations.During accumulating and mature period, only cryptotanshinone at 128μg/mL could reduce the matrix of biofilm and kill bacteria inside biofilm (P<0.05), the microstructure was damaged by cryptotanshinone at concentration of 128μg/mL, while 32g/mL of cryptotanshinone had no obvious inhibitory effect(P>0.05).Conclusion Cryptotanshinone has a certain inhibitory effect on different stages of S.epidermidis biofilm, and there is a certain dose effect.关键词
表皮葡萄球菌/生物膜/隐丹参酮Key words
Staphylococcus epidermidis/biofilm/cryptotanshinone分类
医药卫生引用本文复制引用
易辉,祖瑞铃,易玉玲,李燕..隐丹参酮对表皮葡萄球菌生物膜的抑制作用[J].中国感染控制杂志,2017,16(9):798-803,6.基金项目
四川省科技厅应用基础项目(2015JY0159) （2015JY0159）
