中国免疫学杂志2017，Vol.33Issue(9)：1281-1285,1290,6.DOI:10.3969/j.issn.1000-484X.2017.09.001
基于载体减毒单增李斯特菌的弧菌疫苗菌株构建
Construction of vibrio vaccine strain and expression of antigen gene:attenuated Listeria monocytogenes as vaccine vector
摘要
Abstract
Objective:To provide a potential platform for transferring specific antigen against fish bacterial diseases based on attenuated Lm (EGDe-ΔactA/ΔinlB).Methods: Attenuated Lm (EGDe-ΔactA/ΔinlB) was used to express outer membrane protein K (Ompk),a conserved and effective vaccine candidate in vibrio.The identification of recombinant strains and detection of antigen genes were operated with PCR and RT-PCR,respectively.Results: The results of PCR showed that Lm-Ompk (L-O),Lm-Lmo0576-Ompk (L-L-O) and Lm-P-Ompk (L-P-O) were constructed successfully.The identity of foreign gene was 100％ compared with sequence of NCBI.The analysis of transcription showed that the expressions of Ompk in L-O,L-L-O and L-P-O were significant (P<0.001).Moreover,the expression of Ompk in the condition of antibiotic was higher than that in the BHI without antibiotic (P<0.05).Conclusion: Lm-Ompk (L-O),Lm-Lmo0576-Ompk (L-L-O) and Lm-P-Ompk (L-P-O) were constructed successfully.关键词
减毒单增李斯特菌/疫苗载体/弧菌疫苗/Ompk/抗原基因Key words
Attenuated Listeria monocytogenes/Vaccine vector/Vibrio vaccine/Ompk/Antigen gene分类
生物科学引用本文复制引用
丁承超,陈国薇,谢曼曼,郭亮,李杰,孙静娟,刘箐..基于载体减毒单增李斯特菌的弧菌疫苗菌株构建[J].中国免疫学杂志,2017,33(9):1281-1285,1290,6.基金项目
本文受国家自然科学基金面上项目(31371776)和上海市研究生教育创新计划资助. （31371776）
