中国免疫学杂志2017，Vol.33Issue(9)：1392-1397,6.DOI:10.3969/j.issn.1000-484X.2017.09.024
长链非编码H19靶向调节miR-107通过Notch通路对肺癌的侵袭迁移的影响
Long non-coding RNA H19 promoted lung cancer cells migration and invasion through miR-107
李纪远 1张灿斌 1马新 2王强 1王献 1郑帅玉1
作者信息
- 1. 河南科技大学第一附属医院胸外科,洛阳471000
- 2. 河南科技大学第一附属医院风湿科,洛阳471000
- 折叠
摘要
Abstract
Objective:To investigate the effect and the related mechanism of H19 on the invasion and migration ability of pancreatic cancer cells.Methods: The expression of H19 in tissues of pancreatic carcinoma and non-carcinoma adjacent tissues of pancreatic carcinoma were detected.qPCR was used to detect the expression of H19 in different pancreatic cancer cells.The migration and invasion ability of pancreatic cancer cells was detected after silencing H19 using wound healing assays and Transwell matrigel invasion assays.Dual-Luciferase Reporter Assay System was used to detect miR-107regulating H19.The expression of miR-107 in tissues of pancreatic carcinoma and non-carcinoma adjacent tissues of pancreatic carcinoma were detected.The regulation of miR-107 on the migration and invasion ability of pancreatic cancer cells were detected after silencing H19 using wound healing assays and Transwell matrigel invasion assays.Subcutaneous tumor was used to detect the size and volume of the tumor after inject the tumor cells.Immunohistochemistry was used to detect the expression of Ki67 and PCNA.Results: Compared with non-carcinoma adjacent tissues of pancreatic carcinoma,the expression of H19 of tissues of pancreatic carcinoma was significantly increased.The expression of H19 in pancreatic cancer cell A549 was highest.Silencing H19 could inhibit the migration of human pancreatic cancer A549 cells.miR-107 was the direct target of H19.Compared with non-carcinoma adjacent tissues of pancreatic carcinoma,the expression of H19 of tissues of pancreatic carcinoma was significantly decreased.After silencing H19,inhibiting the expression of miR107 could inhibit the migration of human pancreatic cancer A549 cells.Compared with the group of H19-siRNA,H19-siRNA+miR-107-inhibitor group mice tumor volume and weight were significantly bigger.Immunohistochemistry showed that compared with H19-siRNA group,the expression Ki67 and PCNA expression in H19-siRNA+miR-107-inhibitor group increased.Conclusion: H19 plays the role of tumor promotor factor in pancreatic cancer.H19 can affect the invasion and migration ability of pancreatic carcinoma cells by regulating miR-107.关键词
H19/肺癌/miR-107/侵袭试验Key words
H19/Pancreatic cancer/miR-107/Transwell分类
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李纪远,张灿斌,马新,王强,王献,郑帅玉..长链非编码H19靶向调节miR-107通过Notch通路对肺癌的侵袭迁移的影响[J].中国免疫学杂志,2017,33(9):1392-1397,6.
