中国普通外科杂志2017，Vol.26Issue(8)：987-992,6.DOI:10.3978/j.issn.1005-6947.2017.08.006
白细胞介素13对胆管成纤维细胞TGF-β1/Smads通路表达的影响及地塞米松的干预作用
Influence of interleukin 13 on activity of TGF-β1/Smads signaling pathway in bile duct fibroblasts and the interventional effect of dexamethasone
摘要
Abstract
Objective:To investigate the influence of interleukin 13 (IL-13) on activity of transforming growth factor-β1 (TGF-β1)/Smads signaling pathway in bile duct fibroblasts and the interventional effect of dexamethasone (Dex).Methods:Rabbit bile duct fibroblasts were isolated and cultured and then identified.Then,the bile duct fibroblasts were exposed to IL-13 or IL-13 plus different concentrations (0.01,0.05 and 0.25 mg/mL) of Dex respectively for 48 h,using untreated bile duct fibroblasts as blank control.Afterwards,cell proliferation was assessed by CCK-8,the mRNA expressions of TGF-β1,Smad3 and Smad4 were determined by real-time PCR and the protein expressions of TGF-β1 and Smad4 were examined by Western blot.Results:In bile duct fibroblasts after exposure to IL-13 for 48 h,the cell proliferation was significantly increased,the mRNA expressions of TGF-β1,Smad3 and Smad4 and the protein expressions of TGF-β1 and Smad4 were significantly up-regulated (all P＜0.05),and the above changes exerted by IL-13 were significantly inhibited by Dex addition in a certain concentration-dependent manner (part P＜0.05).Conclusion:IL-13 can enhance the activity of TGF-β1/Smads pathway in bile duct fibroblasts,and weakening the activation of this signaling pathway may be one of the mechanisms of the inhibitory effect of Dex on benign biliary stricture.关键词
胆道/缩窄,病理性/成纤维细胞/Smad蛋白质类/白细胞介素13/地塞米松Key words
Biliary Tract/Constriction, Pathologic/Fibroblasts/Smad Proteins/Interleukin-13/Dexamethasone分类
医药卫生引用本文复制引用
李克跃,石承先,汤可立,魏国微,刘振华,黎涛,张帅民,徐贤刚..白细胞介素13对胆管成纤维细胞TGF-β1/Smads通路表达的影响及地塞米松的干预作用[J].中国普通外科杂志,2017,26(8):987-992,6.基金项目
贵州省科学技术厅—贵州省人民医院联合基金资助项目(黔科合LH字[2016]7146). （黔科合LH字[2016]7146）
