中国全科医学2017，Vol.20Issue(27)：3372-3377,6.DOI:10.3969/j.issn.1007-9572.2017.00.037
西藏移居内地的藏族和汉族不适症状大学生血清蛋白表达分析
Serum Protein Expression in Tibetan and Han Chinese College Students with Unwell Symptoms after Migration to the Inland Region of China from Tibet
摘要
Abstract
Background As the people living in the plateau move back and forth between the plateau and the plain more and more frequently,the adaptation of the plateau hypoxia population to the oxygen enriched environment of the plain has gradually attracted wide attention.Objective To analyze the serum protein expression in Tibetan and Han Chinese college students with unwell symptoms who migrated to the inland region of China from Tibet based on serum proteomics,in order to provide an accurate and simple method for early diagnosis of the diseases due to maladjustment in such a population,and to offer molecular targets for the related clinical treatment.Methods The participants met the inclusion criteria were 32 Tibetan and Han college students〔Tibetan group(n=16) and Han group(n=16)〕 with unwell symptoms who enrolled in Xizang Minzu University during the periods of October to November 2013,October to November 2014 and October to November 2015.Serum samples were collected and the serum protein-derived polypeptide was captured by magnetic bead-based weak cation-exchange chromatography(MB-WCX).The expression of serum proteins was analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) technique and compared between the two groups.Results MALDI-TOF-MS analysis of single sample found that,the polypeptide curves of serum protein with molecular weight of 1 000-10 000 m/z in the 3 samples from the same individual in the Tibetan group tended to be consistent,and similar results were obtained in the Han group;there were significant differences in serum protein levels between Tibetan group and Han group.The results of the MALDI-TOF-MS analysis of overall sample showed that there were significant differences in expression levels of serum protein with the molecular weight of 1 000-10 000 m/z between the Tibetan group and Han group;the analysis of its protein polypeptide components showed that a phenomenon of mutual aggregation and less overlap;there were significant differences in the peak expression of serum proteins with molecular weights of 905.65,1 062.10,1 078.11,2 138.79,2 313.40,4 094.67,4 172.89,4 213.96,5 910.71 m/z between Tibetan group and Han group(P<0.05).Conclusion There are some differences in the expression of serum proteins between Tibetan and Han college students with unwell symptoms after migration to the inland region of China from Tibet,and the differences are significant in the expression of serum proteins with molecular weights of 4 094.67,2 138.79 and 905.65 m/z,which can be used as a basis for further identification and localization of the sequences and genes of differential proteins.关键词
社会适应/蛋白质组学/质谱分析法/藏族/汉族/学生/西藏移居内地Key words
Social adjustment/Proteomics/Mass spectrometry/Zang nationality/Han nationality/Students/Migrating to the inland region of China from Tibet分类
医药卫生引用本文复制引用
李文华,李玉香,张新龙,郭宜娇..西藏移居内地的藏族和汉族不适症状大学生血清蛋白表达分析[J].中国全科医学,2017,20(27):3372-3377,6.基金项目
国家自然科学基金资助项目(81360299) （81360299）
西藏自治区自然科学基金资助项目(2016ZR15-17) （2016ZR15-17）
