中国全科医学2017，Vol.20Issue(27)：3400-3404,5.DOI:10.3969/j.issn.1007-9572.2017.07.y18
黑枸杞水提物对中波紫外线辐射后人角质形成细胞增殖与凋亡及凋亡相关蛋白表达水平的影响研究
Effects of Black Wolfberry Water Extracts on Proliferation and Apoptosis and Apoptosis-related-protein Expression Level of HaCaT Cells after UVB Radiation
摘要
Abstract
Objective To research the effect of black wolfberry water extracts on proliferation and apoptosis and P38 mitogen activated protein kinase(P38MAPK), P53, cysteine proteinase 8(caspase-8), cysteine proteinase 3(caspase-3) and B cell lymphoma factor 2(Bcl-2) expression of HaCaT cells after ultraviolet B(UVB) radiation,in order to explore the mechanism of black wolfberry water extracts reducing the damage of HaCaT cells after UVB radiation.Methods From March 2015 to January 2016, black wolfberry water extracts was extracted and HaCaT cells were cultured in vitro.HaCaT cells in logarithmic growth phase were collected and randomly divided into 3 groups by random number table method:control group(without radiation), UVB group(30 mJ/cm2 UVB radiation for 40 min) and black wolfberry water extracts group(30 mJ/cm2 UVB radiation for 40 min+black wolfberry water extracts 2 mg/ml).Cell proliferation in each group was detected by MTS, apoptotic rate in each group was assessed by flow cytometry.The expression levels of P38MAPK, P53, caspase-8, caspase-3 and Bcl-2 in each group were detected by Western blotting.Results Cell proliferation in UVB group was lower than that in control group(P<0.05);cell proliferation in black wolfberry water extracts group was lower than that in control group but higher than that in UVB group(P<0.05).Apoptotic rate in UVB group was higher than that in control group(P<0.05);apoptotic rate in black wolfberry water extracts group was higher than that in control group but lower than that in UVB group(P<0.05).The expression levels of P38MAPK, P53, caspase-8, caspase-3 in UVB group were higher than those in control group, but the expression level of Bcl-2 in UVB group was lower than that in control group(P<0.05);the expression levels of P38MAPK, P53, caspase-8, caspase-3 in black wolfberry water extracts group were lower than those in UVB group, but the expression level of Bcl-2 in black wolfberry water extracts group was higher than that in UVB group(P<0.05).Conclusion The black wolfberry water extracts can promote the proliferation of HaCaT cells after UVB radiation and prevent their apoptosis.The mechanism might be that the black wolfberry water extracts can down-regulate the expression levels of P38MAPK, P53, caspase-8 and caspase-3 and up-regulate the expression level of Bcl-2, so as to reduce the radiation damage of HaCaT cells.关键词
枸杞△/紫外线/细胞增殖/细胞凋亡/P38丝裂原活化蛋白激酶/肿瘤抑制蛋白质p53/半胱氨酸蛋白酶类/B细胞淋巴瘤因子2Key words
Lycium barbarum/Ultraviolet rays/Cell proliferation/Apoptosis/P38 mitogen activated protein kinase/Tumor suppressor protein p53/Cysteine proteases/B cell lymphoma factor 2分类
医药卫生引用本文复制引用
加杨娥,任立汆,燕华玲..黑枸杞水提物对中波紫外线辐射后人角质形成细胞增殖与凋亡及凋亡相关蛋白表达水平的影响研究[J].中国全科医学,2017,20(27):3400-3404,5.基金项目
青海省科技计划应用基础研究项目(2014-ZJ-756) （2014-ZJ-756）
