中国全科医学2017，Vol.20Issue(27)：3426-3430,5.DOI:10.3969/j.issn.1007-9572.2017.27.020
五子衍宗方对环磷酰胺致肝损伤的保护作用及其机制研究
Protective Effect and Mechanism of Wuzi-Yanzong Prescription Against the Liver Injury Induced by Cyclophosphamide
摘要
Abstract
Objective To study the protective effect and mechanism of Wuzi-Yanzong prescription(WP) against the liver injury induced by cyclophosphamide(CTX),so as to provide a reference for clinical treatment of the disease.Methods From September 2015 to June 2016,40 8-week-old SPF grade male BALB/c mice were randomly divided into normal control group,model control group,WP low-dose group,and WP high-dose group,with 10 mice in each.Flavonoids solution was made by dissolving the flavonoids extracted from WP in 1% CMC-Na.WP low-dose and high-dose groups were respectively intragastrically administered with flavonoids solution 140 mg/kg,280 mg/kg,once a day for 30 days from the 1st day of intervention.For modeling,from the 3rd day of intervention,mice in the control group were given intraperitoneal injection of 0.9% sodium chloride solution,while those in the other groups received intraperitoneal injection of CTX 50 mg/kg,once three days for 10 times.Successful modeling was considered when the levels of serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in the model control group were statistically different from those in the normal control group.Twelve hours after the last time of injection,all the mice were sacrificed by cervical dislocation after taking blood from the eyeball.The serum levels of ALT and AST were detected,the liver was obtained to calculate the liver index,to observe the tissue morphology changes after HE staining and to determine the levels of superoxide dismutase(SOD) and malondialdehyde(MDA) in liver tissues.Results The serum ALT and AST levels in the model control mice were higher than those in the normal control group(P<0.01).The serum ALT and AST levels in the WP low-dose and high-dose groups were lower than those in the model control group(P<0.05).The liver index in the model control group was higher than that in the other 3 groups(P<0.01).In the model control group,the hepatic lobules and hepatic cords were disordered,and the hepatocytes were swollen and accompanied by necrosis and cytoplasm loose.However,in the WP low-dose and high-dose groups,liver lobules arranged neatly,liver cell swelling and cytoplasmic extent were significantly reduced compared with those in the model control group,liver cell morphology was uniform,and arranged in regular.The SOD level of liver tissue in the model control group was lower while the MDA level was higher than in the normal control group(P<0.01).WP low-dose group,WP high-dose group had higher SOD level but lower MDA level than the model control group(P<0.05).Conclusion WP has good protective effects on liver injury caused by CTX and its mechanism is probably related to the scavenging of free radical and increasing of cell anti-oxidative function.关键词
药物性肝损伤/环磷酰胺/药理作用分子作用机制/五子衍宗方Key words
Drug-induced liver injury/Cyclophosphamide/Molecular mechanisms of pharmacological action/Wuzi-yanzong prescription分类
医药卫生引用本文复制引用
刘珍财,赵海霞,陈茜,刘光耀,袁丁,张长城..五子衍宗方对环磷酰胺致肝损伤的保护作用及其机制研究[J].中国全科医学,2017,20(27):3426-3430,5.基金项目
国家自然科学基金资助项目(81573931,81373881,81503334) （81573931,81373881,81503334）
三峡大学人才科研启动基金(KJ2014B067) （KJ2014B067）
