中国现代医学杂志2017，Vol.27Issue(19)：1-6,6.DOI:10.3969/j.issn.1005-8982.2017.19.001
吡非尼酮对大鼠肾间质纤维化的防治作用研究
Effect of Pirfenidone on renal interstitial fibrosis in rats
摘要
Abstract
Objective To investigate the effect of Pirfenidone (PFD) on renal interstitial fibrosis in rat model of unilateral ureteral occlusion (UUO) and to reveal the anti-fibrosis mechanism of PFD.Methods Twenty-four standard male SD rats were randomly divided into three groups:normal control group (N),UUO model group (M) and Pirfenidone treated group (S).The rats of the M and S groups underwent left urethral ligation to make the renal interstitial fibrosis model.The S group was given PFD 250 mg/(kg·d) for gavage,meanwhile the N and M groups were given 1％ sodium carboxymethyl cellulose solution for gavage.Two rats were sacrificed from each group on day 1,3,5 and 7 after UUO operation,the kidney of the obstructive side was taken for further research.In each group,pathological changes were observed by HE staining,qRT-PCR was applied to detect the mRNA expression of connective tissue growth factor (CTGF),Western bolt was applied to detect the protein expressions of TGF-β and α-SMA.Results There was obvious renal interstitial fibrosis in the M group.Compared with the N group,the expressions of TGF-β and α-SMA proteins and the mRNA expression of CTGF were significantly increased in the M group(P ＜ 0.05).Compared with the M group,the expressions of above parameters in the S group were decreased significantly (P ＜ 0.05).The level of TGF-β was positively correlated with the level of α-SMA (P ＜ 0.05).Conclusions Pirfenidone has anti-fibrosis effect on rat UUO model by inhibiting renal TGF-β and CTGF expressions,which may control the fibroblast transformation and reduce the extracellular matrix deposition in the renal tissue.关键词
吡非尼酮/单侧输尿管梗阻/肾间质纤维化/结缔组织生长因子/转化生长因子-β/α-平滑肌肌动蛋白Key words
Pirfenidonde/unilateral ureteral obsrtruction/renal interstitial fibrosis/connective tissue growth factor/tranforming growth factor beta分类
医药卫生引用本文复制引用
徐建华,肖红波,程玉花,王瑜,张桂玲,吕金雷,邵毅..吡非尼酮对大鼠肾间质纤维化的防治作用研究[J].中国现代医学杂志,2017,27(19):1-6,6.基金项目
国家自然科学基金(No:81400372,81660129) （No:81400372,81660129）
全国临床医药研究专项基金(No:L2012052) （No:L2012052）
广东省自然科学基金(No:S2013040015040) （No:S2013040015040）
江西省自然科学基金青年基金(No:20132BAB215004,20114BAB215036) （No:20132BAB215004,20114BAB215036）
