中国现代医学杂志2017，Vol.27Issue(19)：7-11,5.DOI:10.3969/j.issn.1005-8982.2017.19.002
利拉鲁肽1对非酒精性脂肪肝大鼠及细胞因子的影响
Effect of Liraglutide on cytokines and rats with nonalcoholic fatty liver disease
摘要
Abstract
Abstrct:Objective To investigate the effect of Liraglutide on cytokines and biochemical indexes in rats with nonalcoholic fatty liver disease.Methods Thirty-two male SD rats were randomly divided into normal control group (NC,10 rats),high fat group (HF,12 rats),HF+Lira group (10 rats).The HF group and the HF+Lira group were given high-fat diet for 16 weeks.After 12 weeks of high-fat feeding in the HF+Lira group,Liraglutide was administered 600 μg/(kg·d) by intraperitoneal injection for 4 weeks.At the end of the 16th week,the rats were sacrificed.The pathological changes of the liver were observed under optical microscope.The serum level of total triglyceride (TG) was detected by automatic biochemical analyzer.The serum levels of interleukin-18 (IL-18),IL-10 and fasting insulin (FINS) were determined by ELISA,and insulin resistance index (HOMA-IR) was assessed by homeostasis mode assessment.TG content of the liver was measured by GPO-PAP method.The F test and SNK-q test were used for statistical analyses.Results Compared with the NC group,the liver function indexes,the serum levels of IL-18 and TG,FBG,FINS and HOMA-IR in the HF group were obviously increased (P ＜ 0.05).Compared with the HF group,the liver function indexes,the serum levels of IL-18 and TG,FBG,FINS and HOMA-IR in the HF+Lira group were all obviously lowered (P ＜ 0.05).The serum level of IL-10 in the HF group was significantly lower than that in the NC group (P ＜ 0.05);the level of IL-10 in the HF+Lira group was significantly higher than that in the HF group (P ＜ 0.05).HOMA-IR,liver steatosis and hepatic lobular inflammation were positively correlated with the serum level of IL-18 (P ＜ 0.05),but negatively correlated with the serum level of IL-10 (P ＜ 0.05).Conclusions Liraglutide can regulate the balance of inflammatory cytokines in rats with nonalcoholic fatty liver disease,alleviate insulin resistance and inflammation,reduce lipid deposition in liver,thus has therapeutic effect on nonalcoholic fatty liver disease.关键词
非酒精性脂肪肝/胰岛素抵抗/利拉鲁肽/白细胞介素18/白细胞介素10Key words
nonalcoholic fatty liver/insulin resistance/Liraglutide/IL-18/IL-10分类
医药卫生引用本文复制引用
侯洪涛,冯佳,裘艳梅,胡义亭,王玉珍..利拉鲁肽1对非酒精性脂肪肝大鼠及细胞因子的影响[J].中国现代医学杂志,2017,27(19):7-11,5.基金项目
河北省卫生计生委基金资助项目(No:ZL20150117) （No:ZL20150117）
