中国现代医学杂志2017，Vol.27Issue(19)：12-16,5.DOI:10.3969/j.issn.1005-8982.2017.19.003
IgA肾病大鼠肾组织中T细胞免疫球蛋白与黏蛋白域基因1的表达和意义
E xpression of TIM-1 in renal tissue of IgAN rats and significance
摘要
Abstract
Objective To investigate the expression of T-cell immunoglobulin domain and mucin domain-1 (TIM-1) in rats with IgA nephropathy (IgAN),and to explore the role of TIM-1 in the pathogenesis of IgAN.Methods Twenty male SD rats were randomized into two groups,i.e.IgAN model group (10 rats) and control group (10 rats).The rats were sacrificed after the completion of building the model and the kidneys were obtained.The pathological changes of the rat kidneys were observed after PAS and immunofluorescence staining.Immunohistochemical staining was used to detect the expression of TIM-1 protein and RT-PCR was used to measure the expression of TIM-1 mRNA in the renal tissue.Results TIM-1 expression in the IgAN group mainly distributed in glomeruli and renal tubules,the expression levels of TIM-1 mRNA and protein significantly increased in the IgAN group compared with those in the control group (P ＜ 0.05).A positive correlation was found between the expression quantity of TIM-1 mRNA and Katafuchi score (r =0.846,P ＜ 0.05).Conclusions TIM-1 may be involved in the occurrence and development of IgAN.关键词
肾小球肾炎/IgA/T细胞免疫球蛋白与黏蛋白域基因-1Key words
IgAN/T-cell immunoglobulin domain and mucin domain-1 (TIM-1)/rat分类
医药卫生引用本文复制引用
王缨,胡贵荣,李弼民..IgA肾病大鼠肾组织中T细胞免疫球蛋白与黏蛋白域基因1的表达和意义[J].中国现代医学杂志,2017,27(19):12-16,5.基金项目
江西省自然科学基金(No:20142BAB205006) （No:20142BAB205006）
江西省教育厅科学技术研究项目(No:14005) （No:14005）
