中国现代医学杂志2017，Vol.27Issue(19)：22-27,6.DOI:10.3969/j.issn.1005-8982.2017.19.005
MIF基因沉默对肝癌细胞系增殖凋亡及ERK/RSK2信号通路的影响
Effect of silencing MIF gene by RNAi on proliferation, apoptosis and ERK/RSK2 signal pathway in hepatic carcinoma cell line
周鸣 1余蕾 2李琴山 2王碧 2杨国珍1
作者信息
- 1. 贵州医科大学医学检验学院,贵州贵阳550025
- 2. 贵州医科大学附属医院贵州省产前诊断中心,贵州贵阳550002
- 折叠
摘要
Abstract
Objective To investigate the effect of silencing MIF gene by RNA interference on apoptosis of hepatic carcinoma cell lines SMMC-7721 and HepG2 and the potential mechanism.Methods The MIF-siRNA interference sequence was transfected into SMMC-7721 and HepG2 cells,Con-siRNA transfected cells were used as the control group.The silencing effect of MIF gene was detected by qRT-PCR and Western blot.The ability of cell proliferation was evaluated by CCK-8 method.The cell apoptosis rate was detected by flow cytometry.The protein levels of Bcl-2,Bax,p53,extracellular signal-regulated kinase (ERK),p-ERK,p90 ribosomal s6 kinase 2 (RSK2),p-RSK2,glycogen synthase kinase 33 (GSK3β),p-GSK3β,Bad and p-Bad were tested by Western blot.Results The expression levels of MIF gene in the cells of the silent groups were significantly lower than that in the control group (P ＜ 0.05).The ability of cell proliferation in the MIF silent groups was obviously lower than that in the control group (P ＜ 0.05).The cell apoptosis rates in the MIF silent groups were significantly higher than that in the control group (P ＜ 0.05).Bax and p53 protein levels in the cells of the MIF silent groups were higher than those of the control group,while the Bcl-2 protein level was lower than that of the control group (P ＜ 0.05).Meanwhile,the protein levels of ERK,RSK2 and Bad were not significantly different between the two MIF silent groups and the control group (P ＞ 0.05).However,the protein levels of GSK3β,p-GSK3β,p-ERK,p-RSK2 and p-Bad were significantly different between the two silent groups and the control group (P ＜ 0.05).Conclusions Silence of MIF gene inhibits the proliferation and promotes the apoptosis of both SMMC-7721 and HepG2 cells maybe via regulation of the ERK/RSK2 signaling pathway.关键词
巨噬细胞移动抑制因子/基因沉默/肝癌细胞系/凋亡/细胞外信号调节激酶Key words
MIF/gene silencing/hepatic carcinoma cell line/apoptosis/ERK分类
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周鸣,余蕾,李琴山,王碧,杨国珍..MIF基因沉默对肝癌细胞系增殖凋亡及ERK/RSK2信号通路的影响[J].中国现代医学杂志,2017,27(19):22-27,6.
