中国现代医学杂志2017，Vol.27Issue(19)：28-33,6.DOI:10.3969/j.issn.1005-8982.2017.19.006
房颤患者Cx40基因单核苷酸多态性研究
Study on Cx40 gene single nucleotide polymorphism in patients with atrial fibrillation
摘要
Abstract
Objective To discuss the correlations of Cx40 gene (GJA5) single nucleotide polymorphism (SNP) with atrial fibrillation.Methods Two hundreds of patients were selected in the Department of Cardiovascular Medicine,the First Affiliated Hospital of University of South China from June 2012 to December 2012 and divided into atrial fibrillation group (98 cases with continuous fibrillation) and control group (102 cases with sinus rhythm).Clinic information of the patients was collected.Left atrial diameters of the patients were collected through chest and cardiac color ultrasonography.DNA was extracted from venous blood.The locus of SNP was detected through PCR and electrophoresis,and directly examined after PCR products were purified.Based on Hardy-Weinberg Equilibrium examination,the genotype and gene frequency of each group were compared.And non-conditional logistic regression analysis was carried out on the risk factors of atrial fibrillation.Results GJA5 gene rs10465885 had A/G polymorphism.The frequency of allele A and G of the atrial fibrillation group was 0.54 and 0.46 respectively,and that of the control group was 0.45 and 0.55 respectively.In accordance with Hardy-Weinberg Equilibrium examination,the groups complied with the equilibrium.The AA genotype of the arial fibrillation group was significantly higher than that of the control group.There was not significant difference in AG,GG genotype or allele frequency between the two groups.The AA genotype of rs10465885 locus of GJA5 gene ((OR) =2.49) and left atrial diameter ((OR) =1.29) were the risk factors of atrial fibrillation.The genotype of GJA5 gene (rs10465885) had no correlation with the left atrial diameter.Conclusions Gene polymorphism exists at rs10465885 locus of GJA5 gene.AA genotype and left atrial diameter are the risk factors of atrial fibrillation.关键词
GJA5基因/rs10465885/基因型/等位基因/心房颤动Key words
GJA5 gene/rs10465885/genotype/allele/atrial fibrillation分类
医药卫生引用本文复制引用
梁志红,刘科成,全智华,唐惠芳..房颤患者Cx40基因单核苷酸多态性研究[J].中国现代医学杂志,2017,27(19):28-33,6.基金项目
国家自然科学基金(No:30900625) （No:30900625）
