中国现代医学杂志2017，Vol.27Issue(19)：40-44,5.DOI:10.3969/j.issn.1005-8982.2017.19.008
过表达KLF4抑制非小细胞肺癌增殖及上皮间质转化的作用机制
Mechanisms of KLF4 over-expression in inhibiting proliferation and EMT of NSCLC
摘要
Abstract
Objective To explore the effect of KLF4 on proliferation and epithelial mesenchymal transition (EMT) innon-small cell lung cancer (NSCLC).Methods KLF4 expression in 100 samples of surgical resected NSCLC and matched normal tumor-adjacent tissues were measured by immunohistochemical staining.KLF4 expression in WI38 and NSCLC cell lines was detected by Western blot.The regulatory effect of KLF4 on proliferation,invasion,migration and EMT markers were confirmed by cell counting,Transwell and Western blot,respectively.Results KLF4 expression was impaired in the NSCLC tissues.Loss of KLF4 expression was correlated with poor clinicopathological features including tumor stage and distant metastasis,upregulated KLF4 attenuated proliferation,invasion and migration.Meanwhile,the expressions of N-cadherin and vimentin were markedly decreased,but Ecaherin and α-catenin expressions were increased after NSCLC cells were transfected with pMXs-hKLF4.Conclusions Our work provides a novel molecular insight into KLF4-directed tumor suppression through regulation of EMT.关键词
KLF4/非小细胞肺癌/增殖/上皮间质转化Key words
KLF4/NSCLC/proliferation/EMT分类
医药卫生引用本文复制引用
刘华松,徐兰兰,张军,郭家龙,林称意,原野,曾敏,程栋梁..过表达KLF4抑制非小细胞肺癌增殖及上皮间质转化的作用机制[J].中国现代医学杂志,2017,27(19):40-44,5.基金项目
湖北省教育厅项目(No:B2016497) （No:B2016497）
