中国药科大学学报2017，Vol.48Issue(4)：445-452,8.DOI:10.11665/j.issn.1000-5048.20170409
紫杉醇温敏脂质体与siRNA金纳米星载药系统的构建与表征
Preparation and characterization of composite delivery system of paclitaxel-loaded temperature sensitive liposome and siRNA-loaded gold nanostar
摘要
Abstract
We prepared gold nanostar (GNS) through seed growth method firstly,then formation of COX-2 siRNA(siCOX-2) and GNS composite modified with polyethylene glyco (PEG),2-amino-2-deoxy-D-glucos (DG) and 9-D-arginin (9R) was prepared.Mterwords,paclitaxel temperature sensitive liposome (PTX-TSL) was prepared by film dispersion method.Finally,siCOX-2 delivery systerm (PTX-TSL-(siCOX-2(9R/DG-GNS)))was obtained by hydrosulfuryl ligand reaction between siCOX-2 (9R/DG-GNS) and PTX-TSL The successful build of siCOX-2 (9R/DG-GNS) was vetified by nuclear magnetic resonance (NMR),sodium dodecyl sulfate polyacryl amide gel electrophoresis (SDS-PAGE),and ultraviolet spectrophotometry and agarose gel electrophoresis method.Particle size of PTX-TSL-(siCOX-2(9R/DG-GNS)) was (292 ± 14) nm and Zeta potential was about -(2.59 ± 0.12) mV,which were measured by Zetasizer Nano ZS90.The morphology of PTX-TSL-(siCOX-2 (9R/DG-GNS)) measured by transmissionelectronmicroscopy showed homogeneous star structure with phospholipid bilayer on the surface,and it showed good thermal conversion efficiency under radiation of 808 nm laser.Differential scanning calorimetry test showed that PTX-TSL phase transition temperature is about 42.6 ℃.The drug loading content(using dialysis method) and encapsulation efficiency of PTX-TSL were about 7.5％ and 95.4％,at the same time,the release process experiment of PTX-TSL showed that it had a good temperature sensitive release performance.It is hopeful that this siCOX-2 system can be used for reducing drug resistance of PTX and improving the treatment effect of PTX through the synergistic antitumor drug resistance effect of siCOX-2.关键词
紫杉醇/温敏脂质体/氨基葡萄糖/金纳米星/siCOX-2Key words
paclitaxel/temperature sensitive liposome/2-amino-2-deoxy-D-glucos/gold nanostar/siCOX-2分类
医药卫生引用本文复制引用
程紫婷,姚珂,刘婉婉,许伯慧,朱红艳..紫杉醇温敏脂质体与siRNA金纳米星载药系统的构建与表征[J].中国药科大学学报,2017,48(4):445-452,8.基金项目
国家自然科学基金资助项目(No.81202467) （No.81202467）
江苏省自然科学基金资助项目(No.BK2012232) （No.BK2012232）
南通大学大学生创新训练计划项目(No.2016129)This research was supported by the Natural Science Foundation Committee of China (No.81202467) （No.2016129）
the Natural Science Foundation of Jiangsu Province (No.BK2012232) （No.BK2012232）
and the College Students Innovative Entrepreneurship Training Program (No.2016129) （No.2016129）
