中国药科大学学报2017，Vol.48Issue(4)：476-482,7.DOI:10.11665/j.issn.1000-5048.20170413
人胰岛素样生长因子-1的体外抗肝纤维化活性
Anti-liver fibrosis activities of human insulin-like growth factor-1 in vitro
摘要
Abstract
This study was focus on investigating the anti-liver fibrosis effects of insulin-like growth factor-1 (IGF-1) in vitro.The effects of IGF-1 on human liver L-02 cell viability and cell cycle were observed.CC14-induced L-02 cell injury was set up to detect the anti-apoptotic activity of insulin-like growth factor-1 (IGF-1).Transforming growth factor β1 (TGF-β1) induced hepatic stellate cell line (HSC-T6) were used as a liver fibrosis model in vitro to analyze the effects of IGF-1 on the expression of liver fibrosis proteins and intracellular TGF-β1/Smad signaling pathway in HSC-T6 cells.The results showed that IGF-1 could relieve the growth inhibition effects of TGF-β1 on L-02 cells,increase the viability of L-02 cells injured by CCl4,decrease the expression of liver fibrosis proteins,and inhibit the TGF-β1/Smad signaling pathway by inhibiting the phosphorylation of Smad3.Our study suggested that IGF-1 exerted anti-liver fibrosis effects by stimulating L-02 cells proliferation,reducing cell damage and inhibiting ECM accumulation via interfering TGF-β1/Smad signaling pathway.关键词
胰岛素样生长因子-1/转化生长因子-β1/肝细胞/肝星状细胞/肝纤维化Key words
insulin-like growth factor-1/transforming growth factor β1/liver cells/hepatic stellate cells/liver fibrosis分类
生物科学引用本文复制引用
万爱妮,徐栋生,蔡燕飞,陈蕴,金坚,李华钟..人胰岛素样生长因子-1的体外抗肝纤维化活性[J].中国药科大学学报,2017,48(4):476-482,7.基金项目
国家高技术研究发展计划(863计划)资助项目(No.2014AA021003) （863计划）
江苏省优势学科建设工程资助项目(PADA)This study was supported by the National High Technology Research and Development Program of China (863 Program) (No.2014AA021003) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PADA) （PADA）
