中国药科大学学报2017，Vol.48Issue(4)：496-502,7.DOI:10.11665/j.issn.1000-5048.20170416
多肽突变体Cbf-14-2抗青霉素耐药细菌的活性研究
Effective antimicrobial activity of Cbf-14-2 against penicillin-resistant bacteria in vitro and in vivo
摘要
Abstract
The antibacterial activity and mechanism of the antimicrobial peptide mutant Cbf-14-2 against NDM-1 carrying recombinant bacteria (E.coli BL21 (DE3)-NDM-1) was investigated in this study.The minimum inhibitory concentration (MIC),minimum bactericidal concentration (MBC) and killing curves (KCs) in vitro were determined by the broth microdilution method.Mice septicemia model was established by interaperitotoneal injection of E.coli BL21 (DE3)-NDM-1 to evaluate the antibacterial activity of this peptide in vivo.Results showed that Cbf-14-2 exhibited a potent antibacterial activity with MIC of 16 μg/mL and killed almost all recombinant bacteria within 120 min.Meanwhile,it significantly improved the survival rate of infected mice up to 70％ with the decreasing of bacterial load in mice lung,liver,spleen and kidney.This powerful clearance ability of Cbf-14-2 against bacteria mainly related to its enhanced membrane penetration ability through neutralizing the negative charges and disrupting the integrity of the bacterial cell membrane.Therefore,Cbf-14-2 is expected to be a potential antimicrobial agent for the treatment of infection induced by multi-drug resistant bacteria,especially for the NDM-1carrying bacteria.关键词
抗菌肽/Cbf-14-2/抗菌活性/NDM-1Key words
antimicrobial peptide/Cbf-14-2/antimicrobial activity/NDM-1分类
医药卫生引用本文复制引用
汪梦筱,马菱蔓,刘含含,蒋美玲,窦洁,周长林..多肽突变体Cbf-14-2抗青霉素耐药细菌的活性研究[J].中国药科大学学报,2017,48(4):496-502,7.基金项目
国家自然科学基金资助项目(No.81673483) （No.81673483）
中央高校基本科研业务费专项资金资助(No.2632017PT03) （No.2632017PT03）
江苏高校优势学科建设工程资助项目(PADA)This study was supported by the National Natural Science Foundation of China (No.81673483) （PADA）
the Fundamental Research Funds for the Central Universities(No.2632017PT03) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PADA) （No.2632017PT03）
