中国药房2017，Vol.28Issue(25)：3536-3540,5.DOI:10.6039/j.issn.1001-0408.2017.25.22
苗药了哥王不同炮制品乙醇提取物对小鼠的急性毒性作用比较
Comparison of Acute Toxicity Effects of Ethanol Extract from Different Processed Products of Miao Medi-cine Wikstroemia indica on Mice
摘要
Abstract
OBJECTIVE:To compare the acute toxicity of ethanol extract from raw product and different processed products of Wikstroemia indica on mice,and provide basis for optimizing the processing technology of perspiration method for W. indica and medication safety. METHODS:Perspiration method was used to process the W. indica pieces for 30 d to get processed product 1 and process its coarse powder for 14,7 d to get processed product 2,processed product 3,respectively. Then using 70％ ethanol as solvent,percolation method was used to extract the raw W. indica and its different processed products,and acute toxicity test was conducted on mice for different ethanol extracts. RESULTS:The median lethal dose(LD50)of ethanol extracts from raw W. in-dica and processed product 1 were 4.05 and 6.65 g/kg,equivalent to 19,32 times of the clinical daily dose of a 70 kg adult,re-spectively. While the LD50 of ethanol extract from processed product 2 and processed product 3 can not be measured,the maximum tolerated dose(MTD)were measured as 20.0,15.0 g/kg,equivalent to 95,71 times of the clinical daily dose of a 70 kg adult,re-spectively;the maximum dose(MLD)were measured as approximately 30.0,20.0 g/kg,equivalent to 143,95 times of the clini-cal daily dose of a 70 kg adult,respectively. CONCLUSIONS:The toxicity of processed products of W. indica is obviously lower than that of raw products,and its toxicity after processing the coarse powder for 14 d is lower than that after processing the coarse powder for 7 d.关键词
了哥王/汗渍法/炮制/急性毒性/小鼠Key words
Wikstroemia indica/Perspiration method/Processing/Acute toxicity/Mice分类
医药卫生引用本文复制引用
冯果,李玮,何新,郑传奇,冷安碧,田小芳..苗药了哥王不同炮制品乙醇提取物对小鼠的急性毒性作用比较[J].中国药房,2017,28(25):3536-3540,5.基金项目
国家中医药管理局中医药部门公共卫生服务项目(No.国中医药科技中药便函〔2015〕132号) （No.国中医药科技中药便函〔2015〕132号）
国家级大学生创新创业训练计划项目(No. 201610662025) （No. 201610662025）
贵阳中医学院基金项目(No.贵中医科院内〔2016〕40号) （No.贵中医科院内〔2016〕40号）
贵州省高校工程技术研究中心建设项目(No.黔教合KY字〔2012〕021号) （No.黔教合KY字〔2012〕021号）
