中国药房2017，Vol.28Issue(25)：3549-3552,4.DOI:10.6039/j.issn.1001-0408.2017.25.25
RGD肽修饰的异长春花碱-粉防己碱脂质体的制备及体外抑瘤作用研究
Preparation of Vinorelbine-tetrandrine Liposomes Modified with RGD and Study on the in vitro Antitumor Effect
摘要
Abstract
OBJECTIVE:To prepare the vinorelbine-tetrandrine liposomes modified with RGD,and study the inhibitory effect on glioma C6 cells. METHODS:Film dispersion method and ammonium sulfate gradient method were used to prepare the vinorel-bine-tetrandrine liposomes modified with RGD,and the morphology and particle size distribution were observed. The vinorelbine content was determined,and sulforhodamine B method was used to respectively determine the inhibitory effects of blank targeting liposomes,normal vinorelbine liposomes and vinorelbine-tetrandrine liposomes modified with RGD on C6 cells. RESULTS:The prepared vinorelbine-tetrandrine liposomes modified with RGD were spherical or almost spherical with smooth surface,and particle size was about 120 nm. The average content of vinorelbine was 28.27 μg/mL(RSD=0.38％,n=3). Blank targeting liposomes had no significant effect on the growth of C6 cells;vinorelbine-tetrandrine liposomes modified with RGD can obviously inhibit the growth of C6 cells,and cell viability after its effect was significantly lower than normal vinorelbine liposomes (P<0.05). CON-CLUSIONS:Vinorelbine-tetrandrine liposomes modified with RGD are successfully prepared,and they show obvious inhibitory ef-fects on the growth of C6 cells.关键词
RGD肽/异长春花碱/粉防己碱/脂质体/体外抑瘤作用Key words
RGD/Vinorelbine/Tetrandrine/Liposomes/in vitro antitumor effect分类
医药卫生引用本文复制引用
居瑞军,王晓敏,晁建平,程岚,李学涛..RGD肽修饰的异长春花碱-粉防己碱脂质体的制备及体外抑瘤作用研究[J].中国药房,2017,28(25):3549-3552,4.基金项目
国家自然科学基金资助项目(No.81673603, 81541081) （No.81673603, 81541081）
辽宁省教育厅重点实验室基础研究项目(No.LZ2015053) （No.LZ2015053）
