中国药理学通报2017，Vol.33Issue(10)：1371-1376,6.DOI:10.3969/j.issn.1001-1978.2017.10.009
17β-雌二醇对乳腺癌细胞迁移的影响及CANP-FN通路的介导作用
Role of calpain-fibronectin signaling inestrogen-induced migration in breast cancer cells
摘要
Abstract
Aim To study the effect of estrogen on the migration of breast cancer cells and the possible underlying mechanisms.Methods Human breast cancer cell lines MCF-7(estrogen receptor, ER+) and MDA-MB-468(ER-) were employed as a model system.Cells were treated with E2 and pretreated with CANP inhibitor(calpeptin)where needed.Wound-healing assay was applied to evaluate cell migration, Western blot assay was performed to observe protein level, and fibronectin expression was silenced by specific siRNA transfection.Results ① Treatmentof MCF-7 and MDA-MB-468 cellswith E2(50 nmol·L-1) increased cell migration by(51.55±5.50) ％(P<0.01)and (40.78±4.78)％(P<0.05), respectively;② E2 significantly up-regulated the expression of FN protein in MCF-7 and MDA-MB-468 breast cancer cells, which was 2.11 times(P<0.05)and 1.86 times(P<0.01), respectively;③ Pretreatment with calpeptin(10 μmol·L-1) decreased E2-induced cell migration by (49.55±6.44)％(P<0.05) in MCF-7 and(36.85±4.40)％ (P<0.01)in MDA-MB-468 cells;④ Calpeptin pretreatment inhibited E2-induced fibronectin up-regulation by(80.12±4.55)％ and(78.84±5.70) ％(P<0.01), respectively;⑤ Knockdown of FN with siRNA suppressed cell E2-induced migration by(40.65±5.80)％(P<0.01)in MCF-7 and(40.88±6.02)％(P<0.05)in MDA-MB-468 cells.Conclusion E2 stimulates the migration of breast cancer cells with or without ER expression and a calpain-FN signaling pathway may be involved in the E2 action.关键词
17β-雌二醇/钙蛋白酶/纤连蛋白/细胞迁移/乳腺癌/雌激素受体Key words
estrogen/calpain/fibronectin/cell migration/breast cancer/estrogen receptor分类
医药卫生引用本文复制引用
于庆龙,张莹,王宏健,金爱,宛蕾,王旭东..17β-雌二醇对乳腺癌细胞迁移的影响及CANP-FN通路的介导作用[J].中国药理学通报,2017,33(10):1371-1376,6.基金项目
国家自然科学基金资助项目(No 31660345,31360252) （No 31660345,31360252）
