中国药理学通报2017，Vol.33Issue(10)：1376-1381,6.DOI:10.3969/j.issn.1001-1978.2017.10.010
金雀异黄酮对三阴乳腺癌MDA-MB-231细胞凋亡及EGFR/PI3K/Akt通路的影响
Effects of genistein on apoptosis and EGFR/PI3K/Akt signal transductionpathway in triple-negative breast cancer MDA-MB-231 cells
摘要
Abstract
Aim To investigate the effect of genistein on apoptosis in triple-negative breast cancer MDA-MB-231 cells and the underlying mechanisms.Methods MTT assay was used to detect the inhibition rate on breast cancer MDA-MB-231 cells of genistein.Hoechst 33258 staining was applied to determine the effect of genistein on morphology of MDA-MB-231 cells.qRT-PCR was employed to detect the mRNA expression of EGFR in MDA-MB-231 cells.Western blot was utilized to determine the expression of Bcl-2, Bax, caspase-3, EGFR, Akt, and p-Akt.The expressions of Akt and p-Akt proteins in breast cancer MDA-MB-231 cells were detected after treated with Akt activator insulin, genistein and in combination with insulin.Results Genistein inhibited the viability of breast cancer MDA-MB-231 cells in a time-dependent manner.The results of Hoechst 33258 staining showed a typical apoptotic morphological changes of MDA-MB-231 cells after treatment of genistein for 36 h.qRT-PCR showed that the mRNA expression of EGFR in MDA-MB-231 cells decreased after treated with genistein for 36 h.The expression levels of Bcl-2, EGFR, Akt, p-Akt, ERK, p-ERK were significantly down-regulated(P<0.01) compared with control.While, the expression of Bax, caspase-3 was significantly up-regulated (P<0.01).It was observed that p-Akt was significantly activated after the treatment of Akt activator insulin (P<0.01), however, significantly down-regulated (P<0.01) when treated with genistein.Conclusion Genistein could inhibit the growth of triple-negative breast cancer MDA-MB-231 cells and induce apoptosis, which probably involves regulating EGFR/PI3K/Akt signaling pathway.关键词
金雀异黄酮/三阴乳腺癌/凋亡/增殖/人表皮生长因子受体/EGFR/PI3K/Akt信号通路Key words
genistein/triple-negative breast cancer/apoptosis/proliferation/epidermal growth factor receptor/EGFR/PI3K/Akt signaling pathway分类
医药卫生引用本文复制引用
韦立群,李婉婷,李通,徐成飞,唐双意,甘嘉亮..金雀异黄酮对三阴乳腺癌MDA-MB-231细胞凋亡及EGFR/PI3K/Akt通路的影响[J].中国药理学通报,2017,33(10):1376-1381,6.基金项目
广西研究生教育创新计划项目(No YCSW2017110) （No YCSW2017110）
广西高校科学技术研究项目(No 2013ZD015) （No 2013ZD015）
