中国药理学通报2017，Vol.33Issue(10)：1387-1392,6.DOI:10.3969/j.issn.1001-1978.2017.10.012
Rho激酶在远距缺血后处理抗心肌缺血/再灌注损伤中的作用
Role of Rho-kinase in remote ischemicpostconditioning against myocardial ischemia/reperfusion injury
摘要
Abstract
Aim To explore the role of Rho-kinase in remote ischemic postcondi-tioning and its possible mechanism.Methods Thirty male Sprague-Dawley rats were divided into five groups(n=6): sham group(Sham), ischemia/reperfusion group(I/R), remote ischemic postconditioning group(RIPostC), I/R with Rho-kinase inhibitor fasudil group(I/R+Fas) and RIPostC with Rho-kinase activator lysophosphatidic acid group(RIPostC+LPA).Throughout the whole process of experiment, mean arterial pressure(MAP), heart rate(HR) and Ⅱ lead electrocardiogram were continuously monitored.At the end of the reperfusion, plasma creatine kinase(CK) and lactate dehydrogenase(LDH) were measured.Myocardial histopathologic changes were observed by hematoxylin and eosin(HE) staining.Infarct size was measured using 2,3,5-triphenyltetrazolium chloride(TTC) staining.The expressions of phospho-myosin light chain(p-MLC) were detected with Western blot analysis.Results Compared with Sham group, the MAP and HR of other groups decreased, while the amplitude of ST segment increased.Compared with I/R group, MAP and HR increased, the amplitude of ST segment decreased, plasma CK and LDH activity decreased, myocardial pathological morphology and infarct size were improved significantly, infiltration of inflammatory cells was reduced, and the expression of p-MLC decreased in RIPostC and I/R+Fas group.Compared with RIPostC group, RIPostC+LPA group attenuated the effects of RIPostC, and the recovery of the above indicators were inhibited.Conclusion Rho-kinase signaling pathway might mediate remote ischemia postconditioning against myocardial ischemia/reperfusion injury.关键词
Rho激酶/远距缺血后处理/缺血/再灌注损伤/心肌保护/法舒地尔/溶血磷脂酸Key words
Rho-kinase/remote ischemic postconditioning/ischemia/reperfusion/cardioprotection/fasudil/lysophosphatidic acid分类
医药卫生引用本文复制引用
闵凤,贾贤杰,时红杰,胡静,胡志远,高琴,于影..Rho激酶在远距缺血后处理抗心肌缺血/再灌注损伤中的作用[J].中国药理学通报,2017,33(10):1387-1392,6.基金项目
安徽省自然科学基金青年项目(No 1508085QH150) （No 1508085QH150）
蚌埠医学院研究生创新计划项目(No Byycxz1632) （No Byycxz1632）
国家级大学生创新项目(No 201610367011) （No 201610367011）
