中国药理学通报2017，Vol.33Issue(10)：1430-1435,6.DOI:10.3969/j.issn.1001-1978.2017.10.020
补体旁路激活致内皮细胞纤溶凝血功能的改变及干预研究
Abnormality of coagulation and fibrinolysis functions of endothelial cells induced by activated complement alternative pathway and intervention
摘要
Abstract
Aim To investigate the change of coagulation and fibrinolysis functions of human microvascular endothelial cells (HMEC) induced by activated complement alternative pathway and effect of pyrrolidine-dithiocarbamate (PDTC) and resveratrol (Res) on intervention.Methods Normal human serum was activated by cobra venom factor (CVF).After exposure of HMEC to activated complement for various time points, supernatant was removed and assayed for activities of hydrolysing chromogenic substrate and affecting activated partial thromboplastin time (APTT) and prothrombin time (PT).The cells exposed to activated complement were collected and washed, and then the cell suspension was assayed for activity of affecting coagulation function of normal plasma.Lastly, the coagulation and fibrinolysis functions of HMEC pretreated with PDTC and Res were also investigated after HMEC was exposed to activated complement alternative pathway.Results The hydrolysis activity of chromogenic substrate of supernatant was up-regulated significantly after HMEC exposed to activated complement alternative pathway.The supernatant induced APTT decreased significantly, and also shortened PT.The cell suspension of various time points induced APTT decreased significantly, and also shortened PT by suspension of 6 h time point.PDTC and Res failed to inhibit the up-regulation of the chromogenic hydrolysis activity, but Res showed significant intervention on decrease of APTT, and PDTC had better effect on inhibiting the decrease of PT than that of Res.Conclusion Activated complement alternative pathway can induce abnormality of coagulation and fibrinolysis functions of HMEC, and PDTC and Res can affect this change.关键词
补体/内皮细胞/纤溶/凝血/PDTC/白藜芦醇/眼镜蛇毒因子Key words
complement/endothelial cells/fibrinolysis/coagulation/PDTC/resveratrol/cobra venom factor分类
中医学引用本文复制引用
李敏,路青瑜,李亚男,孙黔云..补体旁路激活致内皮细胞纤溶凝血功能的改变及干预研究[J].中国药理学通报,2017,33(10):1430-1435,6.基金项目
国家自然科学基金资助项目(No 81260494) （No 81260494）
贵州省高层次创新型人才项目(黔科合人才[2016]4018号) （黔科合人才[2016]4018号）
贵州省科技创新人才团队项目(黔科合平台人才[2016]5625号) （黔科合平台人才[2016]5625号）
