中国药理学通报2017，Vol.33Issue(10)：1452-1456,5.DOI:10.3969/j.issn.1001-1978.2017.10.024
黄芪甲苷在盲肠末端结扎穿孔诱导脓毒症小鼠中的保护作用研究
Study on protective effect of astragaloside Ⅳ in septic mice
摘要
Abstract
Aim To study the effect of astragaloside (AS-Ⅳ) in CLP-induced septic mice.Methods C57BL/6 mice were randomly divided into the sham group, CLP group and CLP+ AS-Ⅳ group.Two days before operation, AS-Ⅳ (10 mg·kg-1) solution was intragastrically administered into CLP +AS-Ⅳ group, and the other groups were treated with normal saline.A sepsis model was established by cecal ligation and puncture (CLP).Blood, peritoneal fluid and tissue organs were collected at 6 h and 24 h.Neutrophils of blood were purified by Percoll density gradient.Transwell was used to detect the chemotaxis function of neutrophils.The killing activity of neutrophils was detected by coculture with E.coli.Results The survival rate of AS-Ⅳ-pretreated septic mice significantly increased.The number of neutrophils in peritoneal fluid was enhanced markedly.The number of bacteria in the peritoneal fluid, blood and tissue organs such as liver, lung and kidney significantly decreased after AS-Ⅳ pretreatment.The chemotaxis and killing activity of neutrophils increased significantly in AS-Ⅳ-treated mice (P<0.05).Conclusion Astragaloside displays an immunoprotective effect in CLP-induced septic mice, which is related to the upregulation of CXCR2 expression on neutrophils and the increase of neutrophil antibacterial activity.关键词
黄芪甲苷/脓毒血症/中性粒细胞/CLP/CXCR2/免疫调节Key words
astragaloside/sepsis/neutrophils/CLP/CXCR2/immunoregulation引用本文复制引用
袁保红,黄萍,邓鑫梦,王柯英,戴良成,尹辉..黄芪甲苷在盲肠末端结扎穿孔诱导脓毒症小鼠中的保护作用研究[J].中国药理学通报,2017,33(10):1452-1456,5.基金项目
广东省医学科学技术研究基金(No A2016226) （No A2016226）
广东省自然科学基金资助项目(No 2014A030313581,2015A030313581) （No 2014A030313581,2015A030313581）
