中国药业2017，Vol.26Issue(17)：10-13,4.DOI:10.3969/j.issn.1006-4931.2017.17.003
高效液相色谱法同时测定山豆根中4种生物碱的含量与聚类分析
Simultaneous Determination of Four Alkaloids in Radix et Rhizoma Sophorae Tonkinensis by HPLC and Cluster Analysis
摘要
Abstract
Objective To establish an HPLC method for simultaneous determination of matrin, oxymatrine, sophocarpine and oxysopho-carpine in Radix et Rhizoma Sophorae tonkinensis. Methods The Agilent Zorbax SB C18 column(150 mm × 4. 6 mm, 5 μm) was adopted with methanol ( A ) -0. 2％ phosphoric acid solution ( B ) ( adjusted pH=5. 0 by triethylamine ) as mobile phase in gradient elution. The flow rate was 1 mL/min and detection wavelength was 215 nm, the column temperature was 30 ℃. Results The linear ranges of ma-trin, oxymatrine, sophocarpine and oxysophocarpine were 31. 40-2910. 47 μg/mL, 28. 70-164. 53 μg/mL, 50. 16-813. 43 μg/mL, 71. 43-4300. 00 μg/mL ( r≥0. 9990 ) , the average percentages of recovery were in the range of 100. 41％ to 101. 23％ with the RSD between 1. 82％ to 2. 16％ ( n=6 ) . Conclusion This method is simple, accurate and repeatable. The content of 4 alkaloids in different habitats is different, and the content of four alkaloids in geo-authentic habitats of Radix et Rhizoma Sophorae tonkinensis was relatively high.关键词
高效液相色谱/山豆根/生物碱/含量测定/聚类分析Key words
HPLC/Radix et Rhizoma Sophorae tonkinensis/alkaloid/content determination/cluster analysis分类
医药卫生引用本文复制引用
程钱,邹秦文,汪建芬,韩红园,刘雯雪,徐柯心,康帅,马志强,林瑞超..高效液相色谱法同时测定山豆根中4种生物碱的含量与聚类分析[J].中国药业,2017,26(17):10-13,4.基金项目
2015年中央公益性行业科研专项"中药饮片质量保障体系研究(一)"[ 201507002 ]. （一）
