中国药业2017，Vol.26Issue(17)：17-20,4.DOI:10.3969/j.issn.1006-4931.2017.17.005
超高效液相色谱法测定炎可宁胶囊中4组分含量
Simultaneous Determination of Four Components in Yankening Capsules by UPLC
摘要
Abstract
Objective To establish an UPLC method for the content determination of phellodendrine chloride, baicalin, emodin and chrysophanol of Yankening Capsules at the same time. Methods The water ACQUITY UPLC BEH C18 column (100 mm × 2. 1 mm, 1. 7 μm ) was adopted with acetonitrile ( A ) -0. 05 mol/L KH2PO4 ( adjusted pH=5. 0 by KOH, B ) as mobile phase in gradient elution, the flow rate was 0. 3 mL/min, the column temperature was 35 ℃, the detection wavelength was set at 244 nm. Results The linear ranges of phellodendrine chloride, baicalin, emodin and chrysophanol were 0. 04930-0. 086276 μg ( r=0. 9990 ) , 0. 032450-0. 648995 μg ( r=0. 9999 ) , 0. 004979-0. 99588 μg ( r=0. 9999 ) , 0. 002486-0. 428280 μg ( r=0. 9998 ) , respectively. The average percentages of recovery were 95. 44％, 95. 20％, 99. 61％ and 95. 60％, respectively. The baseline was separated in each group, the negative sample had no interference, and the target peaks showed good conditions. Conclusion This method can be used for the simultaneous analysis and the quality control of Yankening Capsules.关键词
炎可宁胶囊/超高效液相色谱/盐酸黄柏碱/黄芩苷/大黄素/大黄酚Key words
Yankening Capsules/UPLC/phellodendrine chloride/baicalin/emodin/chrysophanol分类
医药卫生引用本文复制引用
张建华,苏晶,陈卓..超高效液相色谱法测定炎可宁胶囊中4组分含量[J].中国药业,2017,26(17):17-20,4.基金项目
国家十二五重大专项"中药质量安全检测和风险控制技术平台"[ 2014ZX09304307-002 ]. （）
