中国药业2017,Vol.26Issue(17):21-23,3.DOI:10.3969/j.issn.1006-4931.2017.17.006
高效液相色谱法测定肝炎康胶囊中槲皮素含量
Content Determination of Quercetin in Ganyankang Capsules by HPLC
摘要
Abstract
Objective To establish an HPLC method for the content determination of quercetin in Ganyankang Capsules. Methods The Zorbax Eclipse XDB-C18 column ( 250 mm × 4. 6 mm, 5 μm ) was adopted with the mobile phase of methanol-0. 1%H3PO4 ( 53 :47 ) at flow rate of 1. 0 mL/min, the column temperature was 30 ℃ and the detection wavelength was set at 360 nm, the injection volume was 5 μL. Results The linear range of quercetin was 3. 7-236. 8 μg/mL ( r=0. 9999 ) with an average recovery of 99. 79% , RSD=0. 82% ( n=6 ) . Conclusion The method is accurate, specific and reproducible, which can be used for the quality control of quercetin in Ganyankang Capsules.关键词
肝炎康胶囊/槲皮素/高效液相色谱法Key words
Ganyankang Capsules/quercetin/HPLC分类
医药卫生引用本文复制引用
韦亚洁,李平,秦龙..高效液相色谱法测定肝炎康胶囊中槲皮素含量[J].中国药业,2017,26(17):21-23,3.基金项目
甘肃省自然科学基金[ 145RJZA207 ] ()
兰州大学第二医院2015年度院内博士基金[ ynbskyjj2015-2-17 ] ()
兰州大学第二医院院内中医药科研项目[ YJzy2013-26 ]. ()