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高效液相色谱法测定肝炎康胶囊中槲皮素含量

韦亚洁 李平 秦龙

中国药业2017,Vol.26Issue(17):21-23,3.
中国药业2017,Vol.26Issue(17):21-23,3.DOI:10.3969/j.issn.1006-4931.2017.17.006

高效液相色谱法测定肝炎康胶囊中槲皮素含量

Content Determination of Quercetin in Ganyankang Capsules by HPLC

韦亚洁 1李平 1秦龙2

作者信息

  • 1. 兰州大学第二医院,甘肃兰州 730030
  • 2. 甘肃省消化系肿瘤重点实验室,甘肃兰州 730030
  • 折叠

摘要

Abstract

Objective To establish an HPLC method for the content determination of quercetin in Ganyankang Capsules. Methods The Zorbax Eclipse XDB-C18 column ( 250 mm × 4. 6 mm, 5 μm ) was adopted with the mobile phase of methanol-0. 1%H3PO4 ( 53 :47 ) at flow rate of 1. 0 mL/min, the column temperature was 30 ℃ and the detection wavelength was set at 360 nm, the injection volume was 5 μL. Results The linear range of quercetin was 3. 7-236. 8 μg/mL ( r=0. 9999 ) with an average recovery of 99. 79% , RSD=0. 82% ( n=6 ) . Conclusion The method is accurate, specific and reproducible, which can be used for the quality control of quercetin in Ganyankang Capsules.

关键词

肝炎康胶囊/槲皮素/高效液相色谱法

Key words

Ganyankang Capsules/quercetin/HPLC

分类

医药卫生

引用本文复制引用

韦亚洁,李平,秦龙..高效液相色谱法测定肝炎康胶囊中槲皮素含量[J].中国药业,2017,26(17):21-23,3.

基金项目

甘肃省自然科学基金[ 145RJZA207 ] ()

兰州大学第二医院2015年度院内博士基金[ ynbskyjj2015-2-17 ] ()

兰州大学第二医院院内中医药科研项目[ YJzy2013-26 ]. ()

中国药业

OACSTPCD

1006-4931

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