中国药业2017，Vol.26Issue(18)：4-7,4.DOI:10.3969/j.issn.1006-4931.2017.18.002
天麻成分对羟基苯甲醛抗血小板聚集的作用机制研究
Mechanism of Anti-Platelet Aggregation of P-Hydroxybenzaldehyde from Gastrodia Elata
摘要
Abstract
Objective To investigate the mechanism of the anti-platelet aggregation effect of p-Hydroxybenzaldehyde(PHBA) from Gastrodia elata. Methods Enzyme - linked immunosorbent assay(ELISA)was used to detect the effect of PHBA(320,80,20 μmol/L) on the content of cyclic adenosine monophosphate(cAMP) in platelet-rich plasma(PRP) of rats induced by adenosine diphosphate (ADP). The effect of PHBA on the external platelet cytosolic calcium concentration in rabbits induced by ADP was measured by du-al-wavelength Fura-2 fluorescence spectrophotometry. The expression of P2Y12 receptor induced by ADP was detected by western blot after 5 days of continuous administration of PHBA (20,15,10 mg / kg)rats. Results The high and medium concentrations (320, 80 mol/L) of PHBA can significantly increase the levels of cAMP in PRP of rats,which were significantly higher than that in the model group,and the difference was statistically significant (P < 0. 01 or P < 0. 05). Different concentrations of PHBA (320,80, 20 μmol/L)had no obvious effect on intracellular calcium release and external calcium influx in ADP-induced rabbit platelets. High concentration of PHBA (20 mg / kg ) could significantly inhibit the expression of platelet P 2 Y 12 receptor induced by ADP . Conclusion The mechanism of the anti-platelet aggregation effect of PHBA from Gastrodia elata is related to the increasing of platelet cAMP level and the inhibition of platelet P2Y12 receptor expression.关键词
天麻/成分/对羟基苯甲醛/抗血小板聚集/作用机制Key words
Gastrodia elata/p-hydroxybenzaldehyde/anti-platelet aggregation/mechanism分类
医药卫生引用本文复制引用
申婷,肖纯,向彬,郭营营,李秀芳..天麻成分对羟基苯甲醛抗血小板聚集的作用机制研究[J].中国药业,2017,26(18):4-7,4.基金项目
国家自然科学基金[81303263] （）
云南省高层次中医药人才培养项目[10452300500]. （）
