摘要
Abstract
Objective To establish the quality standard for the Antongshu Plaster. Methods TLC was used for the qualitative identifi-cation of Curcuma longa,Euphorbia kansui,HPLC was used for the content determination of Strychnos nux- vomica and Angelica sinensis. The content determination of Strychnos nux- vomica:Ultimate C18 column and Capcell Pak C18 MG column (250 mm × 4. 6 mm, 5 μm)were adopted with acetonitrile -0. 01 mol/L KH2PO4 and 0. 02 mol/L sodium heptanesulfonate mixture solution (pH=2. 8 adjusted with phosphoric acid)(20 :80)as the mobile phase,the flow rate of 1. 0 mL/min,the column temperature was 35 ℃,the detection wavelength was 260 nm. The content determination of Angelica sinensis:The column was the same as above,the mobile phase was methanol - 0. 05 mol/L phosphoric acid solution (26:74),the detection wavelength was 319 nm,the column temperature was 35 ℃. Results The strychnine,brucine and ferulic acid showed a good linear in the range of 0. 02492-0. 4384 μg,0. 02392-0. 4784 μg, 0. 0892-0. 8028 μg,respectively,and the regression equations were Y=5. 59824 × 106 X+77520(r=0. 9999),Y=6 × 10-7 X+0. 0075(r=0. 9998),Y=6 × 10-2 X+0. 56387(r=0. 9998),respectively. The average recovery rate of brucine,strychnine and ferulic acid were 99. 97%,96. 87% and 97. 68%,respectively. Conclusion The quality standard is simple and easy to operate. The quali-tative and quantitative methods have high accuracy,good repeatability and reasonable test setting index,which can be used for the qual-ity control of Antongshu Plaster.关键词
薄层色谱法/高效液相色谱法/安痛舒贴/桃仁/当归/甘遂/士的宁/马钱子碱/阿魏酸/含量测定/定性鉴别/质量控制Key words
TLC/HPLC/Antongshu Plaster/Prunus persica/Angelica sinensis/Euphorbia kansui/strychnine/brucine/ferulic acid/content determination/qualitative identification/quality control分类
医药卫生
