中国真菌学杂志2017，Vol.12Issue(4)：198-202,220,6.
Sensititre YeastOne显色药敏板与微量肉汤稀释法检测阿萨希毛孢子菌体外药物敏感性比较研究
Comparative evaluation of Sensititre YeastOne colorimetric panel with broth microdilution methods for antifungal susceptibility testing of Trichosporon asahii isolates
摘要
Abstract
Objective To assess the utility of a commercially prepared,colorimetric microdilution panel (Sensititre YeastOne) for testing T.asahii isolates.Methods A total of 62 T.asahii isolates against seven antifungal agents were detected simultaneously by both Sensititre YeastOne and broth microdilution method.The broth microdilution method was adopted as reference method.MIC endpoints were read after 24 h and 48 h of incubation.Results Susceptibility results by broth microdilution method:caspofungin and micafungin showed no in vitro antifungal activities against T.asahii,both of their MIC90 s were 16 μg/mL.Azoles had good antifungal activities against T.asahii isolates,MIC90 were fluconazole (8 μg/mL),voriconazole (0.125 μg/mL) and itraconazole (0.5 μg/mL) at 24 h incubation.Three isolates (4.8％,3/62) of T.asahii had high MICs (4 μg/mL) against amphotericin B.After 24 h incubation,essential agreement (EA) between broth microdilution method and Sensititre YeastOne in seven agents were fluconazole (93.5 ％),voriconazole (98.4 ％),itraconazole (98.4 ％),amphotericin B (98.4 ％),5-flucytosine (88.7 ％),caspofungin(100 ％),micafungin(100 ％),respectively;EA between the two methods decreased to 83.9 ％ (amphotericin B) and 67.7 ％ (flucytosine) when MICs were determined at 48 h incubation.For broth microdilution method,poor agreement was observed mainly for am photericin B (69.4 ％) and flucytosine (53.2 ％),while for Sensititre YeastOne,the overall EA of 24 h and 48 h MICs were excellent except for flucytosine (11.3 ％).Conclusions Azoles had good in vitro antifungal activities against T.asahii isolates.The Sensititre YeastOne appeared to be comparable to the CLSI broth microdilution method in testing T.asahii isolates and could be used as an alternative for susceptibility testing of T.asahii isolates in clinical laboratories.关键词
阿萨希毛孢子菌/药敏试验/微量肉汤稀释法/Sensititre YeastOneKey words
T.asahii/antifungal susceptibility/broth microdilution/Sensititre YeastOne分类
医药卫生引用本文复制引用
郭莉娜,于淑颖,王贺,徐英春..Sensititre YeastOne显色药敏板与微量肉汤稀释法检测阿萨希毛孢子菌体外药物敏感性比较研究[J].中国真菌学杂志,2017,12(4):198-202,220,6.基金项目
首都卫生发展科研专项项目(2016-1-4013) （2016-1-4013）
