中国卒中杂志2017，Vol.12Issue(9)：808-811,4.DOI:10.3969/j.issn.1673-5765.2017.09.009
三七总皂甙对大鼠海马缺血再灌注损伤的保护作用
Protective Effect of Panax Notoginseng Saponins on Cerebral Ischemia–reperfusion Injury in Rats
摘要
Abstract
Objective To observe the effect of panax notoginseng saponins (PNS) on expression of apoptosis inducing factor (AIF) in hippocampus of rats with cerebral ischemia-reperfusion, and to investigate the mechanism of neuroprotective effect of PNS. Methods A total of 68 male Wista rats were randomly divided into sham operation group, normal saline control group and PNS treatment group, respectively. The saline control group and each PNS group were re-divided into ischemia-reperfusion 0 h, 2 h, 4 h, 6 h subgroups according to the intervention time points. The modified suture method was used to complete the rat middle cerebral artery occlusion (MCAO) model preparation. The suture was pulled out 2 h later, to cause formation of ischemia-reperfusion. After MCAO 2 h reperfusion at 0 h, 2 h, 4 h, 6 h point in time, intraperitoneal injection of low dose PNS (50 mg/kg), moderate dose PNS (100 mg/kg) and high dose of PNS (150 mg/kg) were given to the PNS treatment group; equivalent-volume of normal saline were given to the control groups, respectively. Immunohistochemistry assay was used to detect the expressions of AIF in the hippocampus of ischemic sides in ischemia-reperfusion rat. Results Compared to sham operation group, the number of AIF positive cells in the saline control group and PNS group significantly increased (All P<0.01). Compared to the saline control group, the number of AIF positive cells in PNS group significantly decreased (All P<0.01). Compared to low and moderate dosage group in PNS group, the number of AIF positive cells in high dose group significantly decreased (AllP<0.01). Conclusion The PNS may decrease hippocampus AIF expression in cerebral ischemia–reperfusion rat, which maybe have protective effect through the inhibition of AIF expression.关键词
脑缺血-再灌注/凋亡诱导因子/三七总皂甙/大鼠Key words
Cerebral ischemia-reperfusion/Apoptosis inducing factor/Panax Notoginseng Saponins/Rat引用本文复制引用
耿建红,刘志辉,王燕,韩国胜,李健..三七总皂甙对大鼠海马缺血再灌注损伤的保护作用[J].中国卒中杂志,2017,12(9):808-811,4.基金项目
山东省潍坊市卫计委科研项目(2016wsjs091) （2016wsjs091）
