摘要
Abstract
Objective:To observe the effect of acupuncture on the expressions of inflammatory factors,MUC5AC and MUC19 in the ovariectomized rabbit with xeroma.Methods:60 female New Zealand white rabbits(60 eyes,right eye) were randomly divided into the blank group,the model group,the acupuncture group and the medication group,with 15 rabbits in each group.The xeroma models were established by bilateral oophorectomy in the other three groups except the blank group.Points of BL1,BL2,GB14,TE23,and GB1 were applied in the acupuncture group;Estradiol Benzoate(200 g/kg) was used through subcutaneous injection in the medication group,the intervention was three times a week for four weeks.Tear fluid were collected for the detection of IL-6,TNF-α,LF,MUC5AC and MUC19 before treatment,one week after treatment,two weeks after treatment,and four weeks after treatment respectively;the expressions of MUC5AC and MUC19 in the conjunctival tissues and the morphology changes of lacrimal gland were observed with immunohistochemistry method after treatment.Results:IL-6 and TNF-α were significantly increased,whereas LF,MUC5AC,and MUC19 were significantly decreased in the model group compared to the blank group,and the difference was statistically significant (P<0.01);the levels of IL-6 and TNF-α in tears were decreased,whereas LF,MUC5AC,and MUC19 in tears were increased,the positive expressions of MUC5AC and MUC19 in the conjunctival tissues were increased in the acupuncture group after treatment in comparison with the control group,promoting intra acinar material emptying and lacrimal secretion,which was significantly superior to the medication group.Conclusion:Acupuncture treatment,on one hand,can inhibit activity of IL-6 and TNF-α,and increase LF level to achieve the antibacterial effect of ocular surface;on the other hand,it can promote goblet cells and Meibomian glands to produce and secrete the sticky proteins,enhance the expressions of the sticky protein in tears,and stabilize lacrimal film to restore the normal structure and function of ocular surface.关键词
针刺疗法/去势雌兔/干眼症/黏蛋白MUC5AC/黏蛋白MUC19Key words
Acupuncture therapy/Ovariectomized rabbits/Xeroma/Sticky protein of MUC5AC/Sticky protein of MUC19分类
医药卫生
