摘要
Abstract
Objective To investigate the effect of saikosaponin D on the growth and migration of human gastric cancer cell line SGC-7901.Methods Cultured human gastric cancer SGC-7901 cells were divided into control group and low,medium,high dose groups,which were treated with 0,5,10 and 20 μmol/L saikosaponin D,respectively.The cell proliferation was determined by MTT method at 24 h,48 h and 72 h after treatment;apoptosis was detected by fluorescence TUNEL method,cell migration ability was assayed by Transwell method,the expression of Livin and Norrin mRNA was detected by Real-time PCR,the expression of Norrin,LivinCDC25A,cyclin A2,p21 and cyclin D1 proteins was detected by blot Western blotting.Results Compared to control group,the expression of CDC25A cyclin A2 in low dose group was decreased (P＜0.05),however,there were no changes in cell apoptosis and expression of Norrin,Livin,p21 and cyclin D1 (P ＞0.05),in medium and high dose groups,cell proliferation was significantly inhibited,apoptosis rate was increased (P＜0.05),the expression of Norrin and Livin mRNA was decreased (P＜0.05),the expression CDC25A and cyclin A2 proteins was inhibited in a dose-effective manner (P＜ 0,05),and there were no changed in expression of p21 and cyclin D1 (P ＞0.05).Conclusion Saikosaponin D can inhibit the growth and migration of human gastric cancer SGC-7901 cells,which is associated with down-regulating Livin and Norrin expression,interfering with the cell cycle and promoting cell apoptosis.关键词
胃癌柴胡皂苷D/凋亡/Norrin/Livin/SGC-7901细胞Key words
Gastric cancer/Saikosaponin D/Apoptosis/Norrin/Livin/SGC-7901 cell
