浙江医学2017，Vol.39Issue(15)：1253-1257,5.DOI:10.12056/j.issn.1006-2785.2017.39.15.2016-1451
谷氨酰胺对COPD患者外周血单个核细胞中p38MAPK、IL-8及IL-17影响的临床研究
Effect of glutamine on p38MAPK, IL-8 and IL-17 expression in PBMC of patients with chronic obstructive pulmonary disease
黄美健 1吴伟2
作者信息
- 1. 310009 杭州市第三人民医院呼吸科
- 2. 安徽医科大学杭州临床学院呼吸科
- 折叠
摘要
Abstract
Objective To investigate the effects of glutamine on the p38MAPK,IL-8 and IL-17 expression in peripheral blood mononuclear cells (PBMCs) of patients with chronic obstructive pulmonary disease (COPD).Methods Thirty-two patients with acute exacerbation of COPD(AECOPD) and 32 patients with stable COPD(SCOPD) were enrolled in the study.Venous blood samples were collected and PBMCs were isolated.The patients were equally divided into the Gln intervention group and blank control group.Sixteen healthy subjects served as normal control group.The expression levels of p38MAPK,IL-8 and IL-17 in PBMCs were detected by real-time PCR.Results The expression of p38MAPK,IL-8 and IL-17 in AECOPD and SCOPD blank control groups were higher than those in normal control group (P＜0.05),while the difference was more marked in AECOPD blank control group.The expression of p38MAPK,IL-8 and IL-17 in AECOPD and SCOPD Gln groups were significantly lower than those in AECOPD and SCOPD blank control groups,respectively (P＜0.01,P＜0.05).Conclusion Glutamine may exert an anti-inflammatory role in COPD patients by inhibiting p38MAPK cell signaling pathway and reducing the expression of IL-8 and IL-17.关键词
慢性阻塞性肺疾病/谷氨酰胺/p38丝裂原活化蛋白激酶/白介素-8/白介素-17Key words
Chronic obstructive pulmonary disease/Glutamine/p38MAPK/IL-8/IL-17引用本文复制引用
黄美健,吴伟..谷氨酰胺对COPD患者外周血单个核细胞中p38MAPK、IL-8及IL-17影响的临床研究[J].浙江医学,2017,39(15):1253-1257,5.
