浙江医学2017，Vol.39Issue(16)：1316-1319,后插1,5.DOI:10.12056/j.issn.1006-2785.2017.39.16.2017-154
鱼油脂肪乳剂对脂多糖诱导急性肺损伤大鼠肝脏的保护作用研究
Hepatoprotective effect of fish oil-based lipid emulsion on rats with lipopolysaccharide-induced acute lung injury and its mechanism
摘要
Abstract
Objective To investigate the hepatoprotective effect and mechanism of fish oil-based lipid emulsion (Omegaven) on rats with lipopolysaccharide (LPS)-induced acute lung injury.Methods Sixty juvenile Sprague Dawley rats were divided into blank group,LPS group and Omegaven group with 20 in each.Normal saline was injected through caudal vein for 7d in blank and LPS groups,while 10％ Omegaven was injected in Omegaven group.On the 8th day,rats in blank group received intratracheal administration of normal saline,while those in Omegaven and LPS groups were given LPS.All the animals were sacrificed 8h later,blood samples were taken for ALT,AST and PGE2 measurement and liver and lung tissues were collected for pathological examinations.Results HE staining showed that the structure of hepatic lobules and alveoli was intact,there were no degeneration in hepatic cells,no exudation and inflammatory cell infiltration in alveoli of blank group,while in Omegaven and LPS groups the liver tissue showed mild non-specific inflammation and the lung tissue presented with significant inflammation cell infiltration and hemorrhage.Serum ALT and AST levels in LPS group were higher than those in blank group (both P＜0.05).Compared with LPS group and blank group,ALT and AST in Omegaven group were not significantly changed (all P ＞0.05).Compared with blank and Omegaven groups,PGE2 levels in LPS group increased significantly (both P＜0.05),while there was no significant difference in PGE2 level between Omegaven group and blank group (P ＞0.05).Conclusion Fish oil-based lipid emulsion (Omegaven) can alleviate liver injury induced by LPS,which may be related to the regulation of arachidonic acid metabolism.关键词
鱼油/急性肺损伤/肝功能/花生四烯酸Key words
Fish oil/Acute lung injury/Liver function/Arachidonic acid引用本文复制引用
黄丽密,王凯,梁雅琴,余震,翁华春,钱燕..鱼油脂肪乳剂对脂多糖诱导急性肺损伤大鼠肝脏的保护作用研究[J].浙江医学,2017,39(16):1316-1319,后插1,5.基金项目
温州市科技局计划项目(2015Y0047) （2015Y0047）
