浙江医学2017，Vol.39Issue(16)：1320-1323,4.DOI:10.12056/j.issn.1006-2785.2017.39.16.2016-1676
骨质疏松性骨折的小鼠模型探索
Establishment of a mouse osteoporotic fracture model
摘要
Abstract
Objective To establish a simple and reproducible osteoporotic fracture model in mice.Methods Fifty 12-week C57BL/6J female mice were randomly divided into control group (n=10),sham group (n=20) and model group (n=20).Mice in control group were sacrificed after one week and the serum estrogen levels were measured.Mice in model group underwent bilateral ovariectomy,while sham operation was given to mice in sham group.One month after operation,the left femur was exposed by lateral incision on left thigh of mice,intramedulary nailing was performed from knee joint for internal fixation,the lower middle 1/3 section of the femur was severed under direct vision to make a transverse or short oblique fracture of the femur.At the week 2 and 4 after operation,the mice in both groups were sacrificed in batches (n=10 for each),serum estrogen levels were measured,bone mineral density (BMD) was determined and the callus were examined with Micro-CT.Results The serum estrogen levels in model group were lower than those in sham group at week 2 and 4 after operation (both P＜0.05).There were significant differences in BMD,bone volume and relative volume fraction between sham group and model group at week 2 and 4 after operation (all P＜0.05).Trabecula separation was significantly higher in model group than that in sham group at week 4 after surgery (P＜0.05),while there was no significant difference in week 2 (P ＞0.05).There was no significant difference in structure model index between the two groups (P ＞0.05).Conclusion Ovariectomy and intramedullary nailing is a simple,intuitional and stable method for establishing a open osteoporotic femur fracture model in mice,which may be used in laboratory study for postmenopausal osteoporotic fracture and bone metabolism.关键词
雌性小鼠/去势/骨质疏松性骨折/动物模型Key words
Female mice/Ovariectomy/Osteoporotic fracture/Animal model引用本文复制引用
李伟龙,余霄,毛宾尧,庞清江..骨质疏松性骨折的小鼠模型探索[J].浙江医学,2017,39(16):1320-1323,4.基金项目
浙江省医药卫生科技计划重点项目(2015ZDA026) （2015ZDA026）
