浙江医学2017，Vol.39Issue(16)：1333-1336,1344,5.DOI:10.12056/j.issn.1006-2785.2017.39.16.2017-1225
miRNA表达谱在缺氧缺血性脑病新生儿血浆中的变化及意义
Expression profile of plasma miRNA in neonates hypoxic-ischemic encephalopathy and its clinical significance
摘要
Abstract
Objective To screen the relevant plasma miRNA in neonates hypoxic-ischemic encephalopathy (HIE),and to investigate its clinical significance.Methods A total of 117 neonates with HIE (including 69 mild cases and 48 moderate or severe cases) were enrolled in the study,30 full term healthy neonates served as healthy controls.The expression profile of plasma miRNA in three groups was examined with miRNA microarray,then 6 miRNAs were chosen for further validation by Real-time PCR.The expression of miRNA was compared among these groups,and the relationship between miRNA expression and diagnosis,treatment and prognosis of HIE was analyzed.Results The miRNA microarray revealed that 55 miRNA were differentially expressed in plasma of HIE group,29 miRNA were up-regulated and 26 miRNA were down-regulated.Compared to healthy controls,the expression of miR-4472,miR-5195-3p,miR-6794-5p was increased significantly in HIE group,while the expression of let-7b-5p decreased significantly (all P＜0.01).The expression of miR-4472,miR-5195-3p and miR-6794-5p in moderate or severe HIE group was significantly higher,and the expression of let-7b-5p was lower than those in mild HIE group (all P＜0.01).In mild HIE group and moderate or severe HIE group,the expression miR-4472,miR-5195-3p and miR-6794-5p was decreased,and the expression of let-7b-5p was increased markedly after successful treatment(all P＜0.01).ROC curve showed that the area under the curve (AUC) of these 4 miRNAs were 0.958,0.938,0.986 and 0.969,respectively.The incidence of sequelae in HIE neonates with highly expressed miR-4472 and miR-6794-5p was significantly higher than that with low expression (both P＜0.05).The expression of miR-5195-3p and let-7b-5p was not correlated with prognosis of HIE neonates (both P ＞0.05).Conclusion The expression of miR-4472,miR-5195-3p,miR-6794-5p in HIE group are significantly up-regulated,while let-7b-5p is down-regulated.The miR-4472 and miR-6794-5p might be used as biomarkers for prognosis of HIE.关键词
缺氧缺血性脑病/新生儿/miRNAKey words
Hypoxic-ischemic encephalopathy/Neonate/miRNA引用本文复制引用
张旭,朱雯,何梦藻,侯秋英,沈方方,张佩华,滕懿群..miRNA表达谱在缺氧缺血性脑病新生儿血浆中的变化及意义[J].浙江医学,2017,39(16):1333-1336,1344,5.基金项目
浙江省科技计划项目(2013C33218) （2013C33218）
浙江省人口计生委科研课题项目(JSW2013-B033) （JSW2013-B033）
