照明工程学报2017，Vol.28Issue(4)：83-88,95,7.DOI:10.3969/j.issn.1004-440X.2017.04.015
基于细胞层面表征的室内用白光LED光生物安全测试方法研究
Study on Photobiological Safety Testing Methods of White LED for Indoor Lighting Based on Cellular Level Characterization
摘要
Abstract
LED technology is currently in a period of rapid development.Its application areas are gradually expanding from the field of outdoor lighting to the field of indoor lighting, and it is expected to become the main light source for ordinary indoor lighting.Photobiological safety of white LED for indoor lighting has become a frontier issue in the field of building light environment.Firstly, in this study, combined with the early results of the project, phosphor excited white LED is used as the experimental light source, and four kinds of human skins and eye cells are used as experimental cells, then MTT colorimetric assay is used to conduct the detection experiment of photobiological safety evaluation index: cell viability, in different irradiation hours and under different irradiation intensities.Secondly, the original data of the experiment are scientifically screened and analyzed to obtain the effect of different irradiation hours and intensities on cell viability.Finally, a comprehensive, fast and accurate photobiological safety testing method of White LED for indoor lighting is established.关键词
室内照明/白光LED/光生物安全/MTT比色法/细胞活力Key words
indoor lighting/white LED/photobiological safety/MTT colorimetric assay/cell viability分类
信息技术与安全科学引用本文复制引用
王琪,焦洋,宋佳音..基于细胞层面表征的室内用白光LED光生物安全测试方法研究[J].照明工程学报,2017,28(4):83-88,95,7.基金项目
国家自然科学基金青年科学基金项目:"基于细胞层面表征的室内用WLED光生物安全研究" (51408405) （51408405）
天津市应用基础与前沿技术研究计划青年项目:"人工光源LED光生物安全性痕量检测方法研究" (15JCQNJC07500) （15JCQNJC07500）
