摘要
Abstract
Objective: To investigate tHe effects and mecHanism of ursoLic acid on tHe proLiferation and apoptosis of LaryngeaL squamous Hep-2 ceLLs. MetHods: THe LaryngeaL squamous Hep-2 ceLLs in LogaritHmic growtH pHase were divided into five groups: tHe bLank controL group, UA groups (12. 5, 25, 50 μmoL/ L), and CispLatin group (6 μmoL/ L).Twenty- four Hours after tHe intervention, tHe ceLLuLar growtH inHibition rate was caLcuLated by MTT, ceLL cycLe and apoptosis rate were anaLyzed by fLow cytometry, tHe expression of Bax mRNA and bcL-2 mRNA were detected by RT-PCR to caLcuLate tHe ratio of Bax/ bcL- 2, and tHe expression of caspase- 3 protein was detected by western bLotting metHod. ResuLts: Compared to tHe bLank controL group, tHe ceLLuLar growtH inHibition rate of UA groups (25, 50 μmoL/L) and CispLatin group (6 μmoL/ L) were significantLy increased, witH increased G0/ G1 and decreased G2/ M, tHe apoptosis rate was significantLy increased; tHe expression of bcL-2 mRNA was significantLy decreased, wHiLe tHe expression of Bax mRNA was significantLy increased , and tHe ratio of Bax/ bcL-2 were significantLy increased; tHe expression of caspase-3 protein were significantLy eLevated; aLL of tHe differences above were significant(P ＜0. 05, P ＜ 0. 01).ConcLusion: UrsoLic Acid Has inHibitive effects on tHe proLiferation and acceLerating effects on tHe apoptosis of LaryngeaL squamous Hep-2 ceLLs; wHicH is perHaps reLated to its effects of aLtering ceLL cycLe, aLtering tHe expression of apoptosisreLated genes and proteins.关键词
熊果酸/人喉鳞状细胞癌/Hep-2细胞/增殖/凋亡Key words
Ursolic acid/Laryngeal squamous/Hep-2 cell/Proliferation/Apoptosis分类
医药卫生
